eIF3η Antibody (C-5): sc-137214
- eIF3η Antibody (C-5) is a mouse monoclonal IgG1 κ eIF3η antibody, cited in 24 publications, provided at 200 µg/ml
- raised against amino acids 131-300 mapping within an internal region of eIF3η of human origin
- eIF3 eta Antibody (C-5) is recommended for detection of eIF3η of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-eIF3 eta Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eIF3η Antibody (C-5) for WB and IHC(P) applications. These reagents are now offered in bundles with eIF3η Antibody (C-5) (see ordering information below).
eIF3η Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF3 eta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eIF3η antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF3 eta protein plays a crucial role in the initiation of protein synthesis in eukaryotic cells, functioning as a key component of the eukaryotic initiation factor 3 (eIF3) complex, which is the largest of the eIF complexes. This complex is essential for the assembly of the 40S ribosomal subunit, facilitating the recruitment of the initiator tRNA and mRNA, thereby promoting the formation of the pre-initiation complex. Proper functioning of eIF3 eta is vital for maintaining efficient protein synthesis, and dysregulation of this process can lead to various diseases, including cancer, where overexpression of eIF3 proteins has been linked to tumorigenesis. Using eIF3η (C-5) monoclonal antibody in research can provide valuable insights into the mechanisms of translation initiation and its implications in cellular function and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF3η Antibody (C-5) References:
- Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. | Valásek, L., et al. 2004. Mol Cell Biol. 24: 9437-55. PMID: 15485912
- eIF3: a connecTOR of S6K1 to the translation preinitiation complex. | Peterson, TR. and Sabatini, DM. 2005. Mol Cell. 20: 655-7. PMID: 16337588
- Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit. | LeFebvre, AK., et al. 2006. J Biol Chem. 281: 22917-32. PMID: 16766523
- Initiation factor eIF3 and regulation of mRNA translation, cell growth, and cancer. | Dong, Z. and Zhang, JT. 2006. Crit Rev Oncol Hematol. 59: 169-80. PMID: 16829125
- eIF3: a versatile scaffold for translation initiation complexes. | Hinnebusch, AG. 2006. Trends Biochem Sci. 31: 553-62. PMID: 16920360
- Individual overexpression of five subunits of human translation initiation factor eIF3 promotes malignant transformation of immortal fibroblast cells. | Zhang, L., et al. 2007. J Biol Chem. 282: 5790-800. PMID: 17170115
- Reconstitution reveals the functional core of mammalian eIF3. | Masutani, M., et al. 2007. EMBO J. 26: 3373-83. PMID: 17581632
- Measles virus N protein inhibits host translation by binding to eIF3-p40. | Sato, H., et al. 2007. J Virol. 81: 11569-76. PMID: 17686838
- An oncogenic role for the phosphorylated h-subunit of human translation initiation factor eIF3. | Zhang, L., et al. 2008. J Biol Chem. 283: 24047-60. PMID: 18544531
- eIF3: a factor for human health and disease. | Gomes-Duarte, A., et al. 2018. RNA Biol. 15: 26-34. PMID: 29099306
- eIF3 Interacts with Selenoprotein mRNAs. | Hayek, H., et al. 2022. Biomolecules. 12: PMID: 36139107
- eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. | Duan, H., et al. 2023. EMBO J. 42: e112362. PMID: 37155573
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF3η Antibody (C-5) | sc-137214 | 200 µg/ml | $322.00 | |||
eIF3η Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-528876 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF3η Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-521452 | 200 µg Ab, 40 µg BP | $361.00 | |||
eIF3η Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-543185 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF3η Antibody (C-5) AC | sc-137214 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF3η Antibody (C-5) HRP | sc-137214 HRP | 200 µg/ml | $322.00 | |||
eIF3η Antibody (C-5) FITC | sc-137214 FITC | 200 µg/ml | $336.00 | |||
eIF3η Antibody (C-5) PE | sc-137214 PE | 200 µg/ml | $349.00 | |||
eIF3η Antibody (C-5) Alexa Fluor® 488 | sc-137214 AF488 | 200 µg/ml | $364.00 | |||
eIF3η Antibody (C-5) Alexa Fluor® 546 | sc-137214 AF546 | 200 µg/ml | $364.00 | |||
eIF3η Antibody (C-5) Alexa Fluor® 594 | sc-137214 AF594 | 200 µg/ml | $364.00 | |||
eIF3η Antibody (C-5) Alexa Fluor® 647 | sc-137214 AF647 | 200 µg/ml | $364.00 | |||
eIF3η Antibody (C-5) Alexa Fluor® 680 | sc-137214 AF680 | 200 µg/ml | $364.00 | |||
eIF3η Antibody (C-5) Alexa Fluor® 790 | sc-137214 AF790 | 200 µg/ml | $364.00 |