eIF2Bδ Antibody (P-6): sc-9981
- eIF2Bδ Antibody (P-6) is a mouse monoclonal IgG2b κ eIF2Bδ antibody, cited in 3 publications, provided at 200 µg/ml
- raised against full length eIF2Bδ
- eIF2B delta Antibody (P-6) is recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-eIF2B delta Antibody (P-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eIF2Bδ Antibody (P-6) for WB and IHC(P) applications. These reagents are now offered in bundles with eIF2Bδ Antibody (P-6) (see ordering information below).
eIF2Bδ Antibody (P-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects the eIF2B delta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-eIF2Bδ antibody (P-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The eIF2B delta protein plays a crucial role in the regulation of protein synthesis in eukaryotic cells, functioning as a part of the eukaryotic initiation factor 2B (eIF2B) complex, which is essential for the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) on the eIF2 complex. This exchange is vital for the formation of the initiation complex that allows for the proper assembly of the ribosome and the commencement of translation. The eIF2B complex is composed of five subunits: eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ, and eIF2Bε, with the eIF2Bε subunit exhibiting guanine nucleotide exchange factor (GEF) activity. Notably, the presence of all five subunits enhances this GEF activity, highlighting the importance of the complete complex in regulating protein synthesis. Furthermore, the phosphorylation of eIF2 can inhibit the GEF activity of eIF2B, a process that is dependent on the eIF2Bα subunit, thereby linking cellular stress responses to the regulation of protein synthesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF2Bδ Antibody (P-6) References:
- Structure and sequence of the gene encoding the alpha-subunit of rat translation initiation factor-2B. | Flowers, KM., et al. 1995. Biochim Biophys Acta. 1264: 163-7. PMID: 7495858
- The delta-subunit of murine guanine nucleotide exchange factor eIF-2B. Characterization of cDNAs predicts isoforms differing at the amino-terminal end. | Henderson, RA., et al. 1994. J Biol Chem. 269: 30517-23. PMID: 7982969
- Cloning and characterization of cDNAs encoding the epsilon-subunit of eukaryotic initiation factor-2B from rabbit and human. | Asuru, AI., et al. 1996. Biochim Biophys Acta. 1307: 309-17. PMID: 8688466
- Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 631-6. PMID: 8809057
- eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 637-43. PMID: 8929216
- Subunit assembly and guanine nucleotide exchange activity of eukaryotic initiation factor-2B expressed in Sf9 cells. | Fabian, JR., et al. 1997. J Biol Chem. 272: 12359-65. PMID: 9139680
- Eukaryotic initiation factor 2B (eIF2B). | Webb, BL. and Proud, CG. 1997. Int J Biochem Cell Biol. 29: 1127-31. PMID: 9438375
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2Bδ Antibody (P-6) | sc-9981 | 200 µg/ml | $322.00 | |||
eIF2Bδ Antibody (P-6): m-IgGκ BP-HRP Bundle | sc-520573 | 200 µg Ab, 40 µg BP | $361.00 | |||
eIF2Bδ Antibody (P-6): m-IgG2b BP-HRP Bundle | sc-548837 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF2Bδ Antibody (P-6) AC | sc-9981 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF2Bδ Antibody (P-6) HRP | sc-9981 HRP | 200 µg/ml | $322.00 | |||
eIF2Bδ Antibody (P-6) FITC | sc-9981 FITC | 200 µg/ml | $336.00 | |||
eIF2Bδ Antibody (P-6) PE | sc-9981 PE | 200 µg/ml | $349.00 | |||
eIF2Bδ Antibody (P-6) Alexa Fluor® 488 | sc-9981 AF488 | 200 µg/ml | $364.00 | |||
eIF2Bδ Antibody (P-6) Alexa Fluor® 546 | sc-9981 AF546 | 200 µg/ml | $364.00 | |||
eIF2Bδ Antibody (P-6) Alexa Fluor® 594 | sc-9981 AF594 | 200 µg/ml | $364.00 | |||
eIF2Bδ Antibody (P-6) Alexa Fluor® 647 | sc-9981 AF647 | 200 µg/ml | $364.00 | |||
eIF2Bδ Antibody (P-6) Alexa Fluor® 680 | sc-9981 AF680 | 200 µg/ml | $364.00 | |||
eIF2Bδ Antibody (P-6) Alexa Fluor® 790 | sc-9981 AF790 | 200 µg/ml | $364.00 |