eIF2Bβ Antibody (P-4): sc-9979
- eIF2Bβ Antibody (P-4) is a mouse monoclonal IgG1 κ eIF2Bβ antibody, cited in 3 publications, provided at 200 µg/ml
- raised against full length eIF2Bβ of rat origin
- eIF2B beta Antibody (P-4) is recommended for detection of eIF2Bβ of mouse, rat and human origin by WB, IP, IF and ELISA; not recommended for IP
- Anti-eIF2B beta Antibody (P-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eIF2Bβ Antibody (P-4) for WB applications. These reagents are now offered in bundles with eIF2Bβ Antibody (P-4) (see ordering information below).
eIF2Bβ Antibody (P-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the eIF2B beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eIF2Bβ (P-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The eIF2B beta protein plays a crucial role in the regulation of protein synthesis initiation in eukaryotic cells, functioning as a guanine nucleotide exchange factor that facilitates the exchange of GDP for GTP on the eIF2 complex. This process is vital for the formation of the eukaryotic initiation complex, which is essential for the accurate translation of mRNA into proteins. The eIF2B complex is composed of five subunits: eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ, and eIF2Bε, with the eIF2Bε subunit exhibiting GEF activity that is significantly enhanced in the presence of the other subunits. Notably, the phosphorylation of eIF2 can inhibit the GEF activity of eIF2B, a process that is dependent on the eIF2Bα subunit, highlighting the intricate regulatory mechanisms that control protein synthesis and the potential implications for cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF2Bβ Antibody (P-4) References:
- Structure and sequence of the gene encoding the alpha-subunit of rat translation initiation factor-2B. | Flowers, KM., et al. 1995. Biochim Biophys Acta. 1264: 163-7. PMID: 7495858
- The delta-subunit of murine guanine nucleotide exchange factor eIF-2B. Characterization of cDNAs predicts isoforms differing at the amino-terminal end. | Henderson, RA., et al. 1994. J Biol Chem. 269: 30517-23. PMID: 7982969
- Cloning and characterization of cDNAs encoding the epsilon-subunit of eukaryotic initiation factor-2B from rabbit and human. | Asuru, AI., et al. 1996. Biochim Biophys Acta. 1307: 309-17. PMID: 8688466
- Cloning of cDNA for the gamma-subunit of mammalian translation initiation factor 2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 631-6. PMID: 8809057
- eIF2B, the guanine nucleotide-exchange factor for eukaryotic initiation factor 2. Sequence conservation between the alpha, beta and delta subunits of eIF2B from mammals and yeast. | Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 637-43. PMID: 8929216
- Subunit assembly and guanine nucleotide exchange activity of eukaryotic initiation factor-2B expressed in Sf9 cells. | Fabian, JR., et al. 1997. J Biol Chem. 272: 12359-65. PMID: 9139680
- Eukaryotic initiation factor 2B (eIF2B). | Webb, BL. and Proud, CG. 1997. Int J Biochem Cell Biol. 29: 1127-31. PMID: 9438375
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2Bβ Antibody (P-4) | sc-9979 | 200 µg/ml | $322.00 | |||
eIF2Bβ Antibody (P-4): m-IgG Fc BP-HRP Bundle | sc-526531 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF2Bβ Antibody (P-4): m-IgG1 BP-HRP Bundle | sc-531904 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF2Bβ Antibody (P-4) AC | sc-9979 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF2Bβ Antibody (P-4) HRP | sc-9979 HRP | 200 µg/ml | $322.00 | |||
eIF2Bβ Antibody (P-4) FITC | sc-9979 FITC | 200 µg/ml | $336.00 | |||
eIF2Bβ Antibody (P-4) PE | sc-9979 PE | 200 µg/ml | $349.00 | |||
eIF2Bβ Antibody (P-4) Alexa Fluor® 488 | sc-9979 AF488 | 200 µg/ml | $364.00 | |||
eIF2Bβ Antibody (P-4) Alexa Fluor® 546 | sc-9979 AF546 | 200 µg/ml | $364.00 | |||
eIF2Bβ Antibody (P-4) Alexa Fluor® 594 | sc-9979 AF594 | 200 µg/ml | $364.00 | |||
eIF2Bβ Antibody (P-4) Alexa Fluor® 647 | sc-9979 AF647 | 200 µg/ml | $364.00 | |||
eIF2Bβ Antibody (P-4) Alexa Fluor® 680 | sc-9979 AF680 | 200 µg/ml | $364.00 | |||
eIF2Bβ Antibody (P-4) Alexa Fluor® 790 | sc-9979 AF790 | 200 µg/ml | $364.00 |