EHD2 Antibody (G-3): sc-515458

EHD2 Antibody (G-3) is a mouse monoclonal IgG3 kappa light chain antibody that detects EHD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EHD2 (G-3) antibody is available as the non-conjugated form. Eps15 homology domain (EHD)-containing proteins, including EHD2, play a crucial role in the exit of receptors and other membrane proteins from the endosomal recycling compartment, which is vital for maintaining cellular homeostasis and regulating membrane protein levels. EHD2, also known as PAST2, is a 543 amino acid protein characterized by one EF-hand domain and one EH domain, and shows predominant expression in the heart, with lower expression levels in the lung, placenta, and skeletal muscle. The EH domain structure facilitates interactions with various binding partners, including the glucose transporter Glut4 and the endocytotic-associated protein EHBP1. When EHD2 associates with insulin-induced Glut4, EHD2 plays a pivotal role in recruiting Glut4 to the plasma membrane, enabling glucose uptake and thus regulating blood sugar levels. EHD2′s interaction with EHBP1 connects endocytotic processes with actin cytoskeleton dynamics, highlighting EHD2′s importance in both glucose homeostasis and the regulation of endocytosis.

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EHD2 Antibody (G-3) References:

1. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins.  |  Pohl, U., et al. 2000. Genomics. 63: 255-62. PMID: 10673336
2. EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.  |  Guilherme, A., et al. 2004. J Biol Chem. 279: 10593-605. PMID: 14676205
3. EHD2 interacts with the insulin-responsive glucose transporter (GLUT4) in rat adipocytes and may participate in insulin-induced GLUT4 recruitment.  |  Park, SY., et al. 2004. Biochemistry. 43: 7552-62. PMID: 15182197
4. EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling.  |  Braun, A., et al. 2005. Mol Biol Cell. 16: 3642-58. PMID: 15930129
5. C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH?  |  Naslavsky, N. and Caplan, S. 2005. J Cell Sci. 118: 4093-101. PMID: 16155252
6. Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegans.  |  George, M., et al. 2007. BMC Cell Biol. 8: 3. PMID: 17233914
7. Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling.  |  Daumke, O., et al. 2007. Nature. 449: 923-7. PMID: 17914359

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EHD2 Antibody (G-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-EHD2 Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EHD2 Antibody (G-3) Specifications

Alternate Product Name: EHD2

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 414-441

Epitope Target: EHD2

Epitope Species: human

Product Reactivity/Detection: EHD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for EHD2 Antibody (G-3): P: Blocking Peptide

The preferred EHD antibody is EHD Antibody (E-5): sc-271658.