EHD Antibody (E-5): sc-271658

EHD Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects EHD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EHD antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Eps15 homology (EH) domain-containing protein family, which includes EHD1, EHD2, EHD3, and EHD4, plays a crucial role in cellular processes, particularly in endocytosis. EHD proteins are characterized by conserved structural features, including an amino-terminal nucleotide-binding consensus site, a bipartite nuclear localization signal, and a carboxy-terminal EH protein-binding domain with an EF-hand motif. EHD1, the most widely expressed member, shows increased expression in the testis, while EHD2 is predominantly found in the heart, EHD3 is expressed in the brain, kidney, liver, placenta, ovary, and heart, and EHD4 is localized in the heart, placenta, and pancreas. The interaction of EHD proteins with specific ligands is essential for their role in endocytosis, highlighting their importance in cellular signaling and nutrient uptake. Understanding the function and distribution of EHD proteins can provide insights into their potential implications in various physiological and pathological processes, making EHD (E-5) antibody a valuable tool for researchers studying these critical cellular mechanisms.

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EHD Antibody (E-5) References:

1. EHD1--an EH-domain-containing protein with a specific expression pattern.  |  Mintz, L., et al. 1999. Genomics. 59: 66-76. PMID: 10395801
2. Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1).  |  Haider, NB., et al. 1999. Gene. 240: 227-32. PMID: 10564830
3. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins.  |  Pohl, U., et al. 2000. Genomics. 63: 255-62. PMID: 10673336
4. Characterization of EHD4, an EH domain-containing protein expressed in the extracellular matrix.  |  Kuo, HJ., et al. 2001. J Biol Chem. 276: 43103-10. PMID: 11533061
5. A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane.  |  Caplan, S., et al. 2002. EMBO J. 21: 2557-67. PMID: 12032069
6. EHD3: a protein that resides in recycling tubular and vesicular membrane structures and interacts with EHD1.  |  Galperin, E., et al. 2002. Traffic. 3: 575-89. PMID: 12121420

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EHD Antibody (E-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-EHD Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EHD Antibody (E-5) Specifications

Alternate Product Name: EHD

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-450

Epitope Target: EHD1

Epitope Species: human

Product Reactivity/Detection: EHD1-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for EHD Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin