EHD Antibody (E-8): sc-390513
- EHD Antibody (E-8) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 281-307 of EHD of human origin
- EHD Antibody (E-8) is recommended for detection of EHD1, EHD2 and EHD3 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-EHD Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for EHD Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with EHD Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
EHD Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects EHD1, EHD2, and EHD3 proteins of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). EHD Antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Eps15 homology (EH) domain-containing protein family, which includes EHD1, EHD2, EHD3, and EHD4, plays a crucial role in cellular processes such as endocytosis and membrane trafficking. EHD1 is ubiquitously expressed, with particularly high levels in the testis, while EHD2 is predominantly found in the heart, EHD3 is expressed in the brain, kidney, liver, placenta, ovary, and heart, and EHD4 is localized to the heart, placenta, and pancreas. The structural features of EHD proteins, including a nucleotide-binding consensus site, a bipartite nuclear localization signal, and a carboxy-terminal EH protein-binding domain with an EF-hand motif, are essential for their function in cellular signaling and trafficking. EHD proteins facilitate ligand-induced endocytosis, highlighting their importance in regulating cellular uptake and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EHD Antibody (E-8) | sc-390513 | 200 µg/ml | $322.00 | |||
EHD Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-523566 | 200 µg Ab, 40 µg BP | $361.00 | |||
EHD Antibody (E-8) AC | sc-390513 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EHD Antibody (E-8) HRP | sc-390513 HRP | 200 µg/ml | $322.00 | |||
EHD Antibody (E-8) FITC | sc-390513 FITC | 200 µg/ml | $336.00 | |||
EHD Antibody (E-8) PE | sc-390513 PE | 200 µg/ml | $349.00 | |||
EHD Antibody (E-8) Alexa Fluor® 488 | sc-390513 AF488 | 200 µg/ml | $364.00 | |||
EHD Antibody (E-8) Alexa Fluor® 546 | sc-390513 AF546 | 200 µg/ml | $364.00 | |||
EHD Antibody (E-8) Alexa Fluor® 594 | sc-390513 AF594 | 200 µg/ml | $364.00 | |||
EHD Antibody (E-8) Alexa Fluor® 647 | sc-390513 AF647 | 200 µg/ml | $364.00 | |||
EHD Antibody (E-8) Alexa Fluor® 680 | sc-390513 AF680 | 200 µg/ml | $364.00 | |||
EHD Antibody (E-8) Alexa Fluor® 790 | sc-390513 AF790 | 200 µg/ml | $364.00 | |||
EHD (E-8) Neutralizing Peptide | sc-390513 P | 100 µg/0.5 ml | $69.00 |