Date published: 2026-5-1

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eHAND Antibody (D-1): sc-390651

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Datasheets
  • eHAND Antibody (D-1) is a mouse monoclonal mouse IgG1 κ κ provided at 200 µg/ml
  • raised against amino acids 1-45 of eHAND of human origin
  • recommended for detection of eHAND of mouse, rat and human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-390651 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for eHAND Antibody (D-1) for WB applications. These reagents are now offered in bundles with eHAND Antibody (D-1) (see ordering information below).
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    eHAND Antibody (D-1) is a mouse monoclonal IgG1 antibody that detects eHAND in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eHAND, also known as HAND1, HXT, or Thing1, is a crucial member of the basic-helix-loop-helix transcription factor family, playing a significant role in cardiac development and left-right asymmetry of the heart. eHAND is predominantly expressed on the left side of the developing heart and in the systemic ventricle, where eHAND is essential for proper formation of heart chambers during cardiogenesis. eHAND′s structure, characterized by its basic-helix-loop-helix motif, allows binding to specific DNA sequences and regulation of target genes involved in cardiac morphogenesis. Understanding eHAND′s structure provides insights into how transcription factors interact with DNA to orchestrate complex processes of heart development. eHAND has been shown to interact with other proteins, such as dHAND, to form heterodimers that enhance regulatory functions, further emphasizing the importance of protein-protein interactions in cardiac development. anti-eHAND antibody (D-1) is an invaluable tool for researchers studying mechanisms of heart development and related pathologies.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    eHAND Antibody (D-1)

    sc-390651
    200 µg/ml
    $322.00

    eHAND Antibody (D-1): m-IgG Fc BP-HRP Bundle

    sc-540828
    200 µg Ab; 10 µg BP
    $361.00

    eHAND Antibody (D-1): m-IgG1 BP-HRP Bundle

    sc-542397
    200 µg Ab; 20 µg BP
    $361.00

    eHAND Antibody (D-1) X

    sc-390651 X
    200 µg/0.1 ml
    $322.00

    How much sample do you recommend loading on the gel for Western blot detection using eHAND (D-1): sc-390651?

    Asked by: DefinitelyNotMatt
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2026, m_5, d_1, h_10CST
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    Rated 5 out of 5 by from Produced positive Western blot data of eHANDProduced positive Western blot data of eHAND expression in non-transfected and human eHAND transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-03-29
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    eHAND Antibody (D-1) is rated 5.0 out of 5 by 1.
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