eHAND Antibody (F-7): sc-390376
- eHAND Antibody (F-7) is a mouse monoclonal IgG2a κ eHAND antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1-45 of eHAND of human origin
- eHAND Antibody (F-7) is recommended for detection of eHAND of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eHAND Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-390376 X, 200 µg/0.1 ml)
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eHAND Antibody (F-7) for WB applications. These reagents are now offered in bundles with eHAND Antibody (F-7) (see ordering information below).
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eHAND Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects eHAND protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eHAND (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eHAND protein, also known as HAND1, HXT, or Thing1, plays a crucial role in cardiac development as a member of the basic-helix-loop-helix transcription factor family. eHAND is primarily expressed on the left side of the developing heart and in the systemic ventricle, where eHAND is essential for proper formation of heart chambers during cardiogenesis. eHAND protein′s localization ensures correct asymmetrical development of the heart, necessary for proper functioning of the cardiovascular system. eHAND interacts with dHAND (HAND2), predominantly expressed on the right side of the heart, highlighting coordinated action of these transcription factors in establishing left-right cardiac asymmetry. eHAND′s ability to regulate gene expression during heart development underscores eHAND′s importance in both embryonic and fetal stages, making eHAND a significant target for studies related to congenital heart defects and other cardiovascular diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eHAND Antibody (F-7) References:
- A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects. | Yamagishi, H., et al. 1999. Science. 283: 1158-61. PMID: 10024240
- HAND proteins: molecular mediators of cardiac development and congenital heart disease. | Srivastava, D. 1999. Trends Cardiovasc Med. 9: 11-8. PMID: 10189962
- A subclass of bHLH proteins required for cardiac morphogenesis. | Srivastava, D., et al. 1995. Science. 270: 1995-9. PMID: 8533092
- Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND. | Srivastava, D., et al. 1997. Nat Genet. 16: 154-60. PMID: 9171826
- The bHLH factors, dHAND and eHAND, specify pulmonary and systemic cardiac ventricles independent of left-right sidedness. | Thomas, T., et al. 1998. Dev Biol. 196: 228-36. PMID: 9576835
- A signaling cascade involving endothelin-1, dHAND and msx1 regulates development of neural-crest-derived branchial arch mesenchyme. | Thomas, T., et al. 1998. Development. 125: 3005-14. PMID: 9671575
- Molecular cloning of the human Hand1 gene/cDNA and its tissue-restricted expression in cytotrophoblastic cells and heart. | Knöfler, M., et al. 1998. Gene. 224: 77-86. PMID: 9931445
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eHAND Antibody (F-7) | sc-390376 | 200 µg/ml | $322.00 | |||
eHAND Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-523510 | 200 µg Ab, 40 µg BP | $361.00 | |||
eHAND Antibody (F-7): m-IgG2a BP-HRP Bundle | sc-546161 | 200 µg Ab; 10 µg BP | $361.00 | |||
eHAND Antibody (F-7) X | sc-390376 X | 200 µg/0.1 ml | $322.00 | |||
eHAND Antibody (F-7) AC | sc-390376 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eHAND Antibody (F-7) HRP | sc-390376 HRP | 200 µg/ml | $322.00 | |||
eHAND Antibody (F-7) FITC | sc-390376 FITC | 200 µg/ml | $336.00 | |||
eHAND Antibody (F-7) PE | sc-390376 PE | 200 µg/ml | $349.00 | |||
eHAND Antibody (F-7) Alexa Fluor® 488 | sc-390376 AF488 | 200 µg/ml | $364.00 | |||
eHAND Antibody (F-7) Alexa Fluor® 546 | sc-390376 AF546 | 200 µg/ml | $364.00 | |||
eHAND Antibody (F-7) Alexa Fluor® 594 | sc-390376 AF594 | 200 µg/ml | $364.00 | |||
eHAND Antibody (F-7) Alexa Fluor® 647 | sc-390376 AF647 | 200 µg/ml | $364.00 | |||
eHAND Antibody (F-7) Alexa Fluor® 680 | sc-390376 AF680 | 200 µg/ml | $364.00 | |||
eHAND Antibody (F-7) Alexa Fluor® 790 | sc-390376 AF790 | 200 µg/ml | $364.00 |