EGFR Antibody (528): sc-120

EGFR Antibody (528) is a mouse monoclonal IgG2a kappa light chain antibody that detects a cell surface epitope of EGFR of human origin in mouse, rat, and human samples through applications such as WB (non-reducing), IP, IF, and FCM. EGFR monoclonal antibody (528) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. The epidermal growth factor receptor (EGFR) is a critical receptor tyrosine kinase that plays a vital role in cell signaling pathways regulating cell proliferation, survival, and differentiation. Located on the cell surface, EGFR is activated upon binding to its ligands, such as epidermal growth factor (EGF) and transforming growth factor α (TGFα), leading to receptor dimerization and subsequent activation of its intrinsic tyrosine kinase activity. This activation triggers a cascade of downstream signaling pathways, including the RAS-RAF-MAPK and PI3K-AKT pathways, which are essential for various cellular processes, including organ morphogenesis and tissue repair. However, aberrant activation of EGFR is frequently associated with tumorigenesis, as EGFR can lead to uncontrolled cell growth, invasion, and metastasis. The presence of EGFR in various cancers makes EGFR a significant therapeutic target, and EGFR monoclonal antibody (528) detection capabilities are crucial for research and clinical applications aimed at understanding and treating EGFR-related malignancies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EGFR Antibody (528) References:

1. Deletion and tandem duplication of exons 2 - 7 in the epidermal growth factor receptor gene of a human malignant glioma.  |  Fenstermaker, RA. and Ciesielski, MJ. 2000. Oncogene. 19: 4542-8. PMID: 11002427
2. A single physiologic dose of ultraviolet light exposure to human skin in vivo induces phosphorylation of epidermal growth factor receptor.  |  Katiyar, SK. 2001. Int J Oncol. 19: 459-64. PMID: 11494022
3. The basic biology of HER2.  |  Rubin, I. and Yarden, Y. 2001. Ann Oncol. 12 Suppl 1: S3-8. PMID: 11521719
4. Antibodies to the autophosphorylation sites of the epidermal growth factor receptor protein-tyrosine kinase as probes of structure and function.  |  Gullick, WJ., et al. 1985. EMBO J. 4: 2869-77. PMID: 2415353
5. Review on EGFR Inhibitors: Critical Updates.  |  Singh, D., et al. 2016. Mini Rev Med Chem. 16: 1134-66. PMID: 26996617
6. Autophosphorylation and protein kinase C phosphorylation of the epidermal growth factor receptor. Effect on tyrosine kinase activity and ligand binding affinity.  |  Downward, J., et al. 1985. J Biol Chem. 260: 14538-46. PMID: 2997213
7. Expression of epidermal growth factor receptors on human cervical, ovarian, and vulval carcinomas.  |  Gullick, WJ., et al. 1986. Cancer Res. 46: 285-92. PMID: 2998607
8. Biosynthesis of the epidermal growth factor receptor in human squamous cell carcinoma lines: secretion of the truncated receptor is not common to epidermal growth factor receptor-hyperproducing cells.  |  Gamou, S., et al. 1988. Cell Struct Funct. 13: 25-38. PMID: 3131023
9. EGFR: An essential receptor tyrosine kinase-regulator of cancer stem cells.  |  Talukdar, S., et al. 2020. Adv Cancer Res. 147: 161-188. PMID: 32593400
10. Globally Approved EGFR Inhibitors: Insights into Their Syntheses, Target Kinases, Biological Activities, Receptor Interactions, and Metabolism.  |  Abourehab, MAS., et al. 2021. Molecules. 26: PMID: 34771085
11. Epidermal growth factor receptors in lung tumours.  |  Berger, MS., et al. 1987. J Pathol. 152: 297-307. PMID: 3668732
12. Epidermal growth factor receptor (EGFR) and EGFR mutations, function and possible role in clinical trials.  |  Voldborg, BR., et al. 1997. Ann Oncol. 8: 1197-206. PMID: 9496384

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EGFR Antibody (528) has 361 citations in a variety of scientific publications.

Primary antibodies like Anti-EGFR Antibody (528) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EGFR Antibody (528) Specifications

Alternate Product Name: EGFR

Product Clone Name: 528

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: EGFR

Epitope Species: human

Product Reactivity/Detection: EGFR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB (non-reducing), IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 361 citations in various scientific publications

Available Conjugates/Forms for EGFR Antibody (528): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, PE: Phycoerythrin