Eg5 Antibody (F-1): sc-374212

Eg5 Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Eg5 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Eg5 antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Eg5, also known as kinesin-like protein KIF11 or TRIP5, plays a crucial role in eukaryotic cell division as a slow, plus-end-directed microtubule-based motor protein belonging to the BimC kinesin family. Eg5′s primary function is to facilitate bipolar spindle formation, which is essential for accurate chromosome segregation during mitosis. Disruption of Eg5 expression leads to the cessation of centrosome migration, resulting in cells being arrested in mitosis with monoastral microtubule arrays, highlighting Eg5′s importance in maintaining proper cell cycle progression. Additionally, Eg5 undergoes post-translational modifications, including phosphorylation on serine residues during the S phase and on both serine and threonine 927 during mitosis, which are critical for regulating Eg5′s interaction with the spindle apparatus, particularly during early prophase. Furthermore, Eg5 is a member of the thyroid receptor interacting protein family and interacts with the thyroid hormone receptor in the presence of thyroid hormone, indicating Eg5′s potential role in hormone signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Eg5 Antibody (F-1) References:

1. The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle.  |  Mountain, V., et al. 1999. J Cell Biol. 147: 351-66. PMID: 10525540
2. Mechanistic analysis of the mitotic kinesin Eg5.  |  Cochran, JC., et al. 2004. J Biol Chem. 279: 38861-70. PMID: 15247293
3. AURKA, DLGAP5, TPX2, KIF11 and CKAP5: Five specific mitosis-associated genes correlate with poor prognosis for non-small cell lung cancer patients.  |  Schneider, MA., et al. 2017. Int J Oncol. 50: 365-372. PMID: 28101582
4. Novel Insights Into the Phenotypical Spectrum of KIF11-Associated Retinopathy, Including a New Form of Retinal Ciliopathy.  |  Birtel, J., et al. 2017. Invest Ophthalmol Vis Sci. 58: 3950-3959. PMID: 28785766
5. Detection and quantification of a KIF11 mosaicism in a subject presenting familial exudative vitreoretinopathy with microcephaly.  |  Karjosukarso, DW., et al. 2018. Eur J Hum Genet. 26: 1819-1823. PMID: 30181612
6. KIF11 Variants Associated With Novel Renal System Involvement-Two Cases That Expand the Phenotypic Spectrum of Microcephaly With or Without Chorioretinopathy, Lymphedema, or Impaired Intellectual Development.  |  Gonzalez, T., et al. 2025. Am J Med Genet A. 197: e63903. PMID: 39404449
7. HPV16 entry requires dynein for minus-end transport and utilizes kinesin Kif11 for plus-end transport along microtubules during mitosis.  |  Keiffer, TR., et al. 2025. J Virol. 99: e0093724. PMID: 39629998
8. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor.  |  Lee, JW., et al. 1995. Mol Endocrinol. 9: 243-54. PMID: 7776974
9. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo.  |  Blangy, A., et al. 1995. Cell. 83: 1159-69. PMID: 8548803
10. Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome.  |  Nakagawa, T., et al. 1997. Proc Natl Acad Sci U S A. 94: 9654-9. PMID: 9275178
11. Expanding the role of HsEg5 within the mitotic and post-mitotic phases of the cell cycle.  |  Whitehead, CM. and Rattner, JB. 1998. J Cell Sci. 111 (Pt 17): 2551-61. PMID: 9701554
12. Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development.  |  Ferhat, L., et al. 1998. J Neurosci. 18: 7822-35. PMID: 9742151

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Eg5 Antibody (F-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Eg5 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Eg5 Antibody (F-1) Specifications

Alternate Product Name: Eg5

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1029-1057

Epitope Target: Eg5

Epitope Species: human

Product Reactivity/Detection: Eg5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Eg5 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin