Eg5 Antibody (D-7): sc-393311

Eg5 Antibody (D-7) is a mouse monoclonal IgG1 antibody that detects Eg5 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Eg5, also known as kinesin-like protein KIF11 or TRIP5, plays a crucial role in eukaryotic cell division as a slow, plus-end-directed microtubule-based motor protein belonging to the BimC kinesin family. Eg5 (D-7) antibody facilitates bipolar spindle formation, which is essential for accurate chromosome segregation during mitosis. When Eg5 expression is inhibited, centrosome migration is disrupted, leading to a halt in cell division and the formation of monoastral microtubule arrays, which can have significant implications for cellular proliferation and cancer development. Eg5 undergoes post-translational modifications, including phosphorylation on serine residues during the S phase and on both serine and threonine 927 during mitosis, which are critical for regulating interaction with the spindle apparatus, particularly during early prophase. Furthermore, Eg5 is a member of the thyroid receptor interacting protein family and interacts with the thyroid hormone receptor only in the presence of thyroid hormone, highlighting potential role in hormone signaling pathways. Anti-Eg5 antibody (D-7) is an invaluable tool for researchers studying cell division and related processes in mouse models.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Eg5 Antibody (D-7) References:

1. Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility.  |  Song, T., et al. 2015. J Cell Sci. 128: 1001-10. PMID: 25588836
2. The mitotic kinesin KIF11 is a driver of invasion, proliferation, and self-renewal in glioblastoma.  |  Venere, M., et al. 2015. Sci Transl Med. 7: 304ra143. PMID: 26355032
3. In Vitro Maturation of Mouse Oocytes Increases the Level of Kif11/Eg5 on Meiosis II Spindles.  |  Kovacovicova, K., et al. 2016. Biol Reprod. 95: 18. PMID: 27146033
4. Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms.  |  Swarnkar, S., et al. 2018. Sci Rep. 8: 17419. PMID: 30479371
5. Targeting the Id1-Kif11 Axis in Triple-Negative Breast Cancer Using Combination Therapy.  |  Thankamony, AP., et al. 2020. Biomolecules. 10: PMID: 32911668
6. KIF11 Promotes Proliferation of Hepatocellular Carcinoma among Patients with Liver Cancers.  |  Hu, ZD., et al. 2021. Biomed Res Int. 2021: 2676745. PMID: 33490265
7. KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.  |  Wei, D., et al. 2021. Int J Biol Sci. 17: 514-526. PMID: 33613109
8. The oral KIF11 inhibitor 4sc-205 exhibits antitumor activity and potentiates standard and targeted therapies in primary and metastatic neuroblastoma models.  |  Masanas, M., et al. 2021. Clin Transl Med. 11: e533. PMID: 34709738
9. Inhibition of KIF11 ameliorates osteoclastogenesis via regulating mTORC1-mediated NF-κB signaling.  |  Miao, J., et al. 2023. Biochem Pharmacol. 217: 115817. PMID: 37757917
10. The Interaction of Calcium-Sensing Receptor with KIF11 Enhances Cisplatin Resistance in Lung Adenocarcinoma via BRCA1/cyclin B1 pathway.  |  Wang, F., et al. 2024. Int J Biol Sci. 20: 3892-3910. PMID: 39113697

The preferred Eg5 antibody is Eg5 Antibody (F-1): sc-374212.