EF-G2 Antibody (E-10): sc-514242
- EF-G2 Antibody (E-10) is a mouse monoclonal IgG1 κ EF-G2 antibody provided at 200 µg/ml
- raised against amino acids 161-219 mapping within an internal region of EF-G2 of human origin
- EF-G2 Antibody (E-10) is recommended for detection of EF-G2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EF-G2 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EF-G2 Antibody (E-10) for WB applications. These reagents are now offered in bundles with EF-G2 Antibody (E-10) (see ordering information below).
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EF-G2 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects EF-G2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EF-G2 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EF-G2, also known as elongation factor G2, ribosome-releasing factor 2 (mitochondrial), GFM2, or MSTP027, is a crucial component of mitochondrial translation, which is essential for maintaining mitochondrial function and preventing mitochondrial DNA impairment. This 779 amino acid protein belongs to the GTP-binding elongation factor family and EF-G/EF-2 subfamily, and is primarily localized in the mitochondrion. EF-G2 plays a vital role in ribosome recycling by directly interacting with mitochondrial ribosome recycling factor (MRRF) on the large subunit of the ribosome, facilitating ribosome dissociation from mRNA. The gene encoding EF-G2 is located on human chromosome 5q13.3 and exists in five alternatively spliced isoforms, with high expression levels found in adult heart, skeletal muscle, kidney, and both adult and fetal liver. Understanding EF-G2 function is critical, as EF-G2 ensures the efficiency of mitochondrial protein synthesis, which is necessary for proper cellular metabolism and energy production.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EF-G2 Antibody (E-10) References:
- Identification and characterization of two novel human mitochondrial elongation factor genes, hEFG2 and hEFG1, phylogenetically conserved through evolution. | Hammarsund, M., et al. 2001. Hum Genet. 109: 542-50. PMID: 11735030
- The molecular basis for tissue specificity of the oxidative phosphorylation deficiencies in patients with mutations in the mitochondrial translation factor EFG1. | Antonicka, H., et al. 2006. Hum Mol Genet. 15: 1835-46. PMID: 16632485
- [Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition]. | Li, J., et al. 2007. Zhonghua Gan Zang Bing Za Zhi. 15: 437-40. PMID: 17594809
- The A3243G tRNALeu(UUR) MELAS mutation causes amino acid misincorporation and a combined respiratory chain assembly defect partially suppressed by overexpression of EFTu and EFG2. | Sasarman, F., et al. 2008. Hum Mol Genet. 17: 3697-707. PMID: 18753147
- Ribosome shifting or splitting: it is all up to the EF-G. | Christian, B., et al. 2009. Mol Cell. 35: 400-2. PMID: 19716785
- EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis. | Tsuboi, M., et al. 2009. Mol Cell. 35: 502-10. PMID: 19716793
- A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi. | Suematsu, T., et al. 2010. Mol Microbiol. 75: 1445-54. PMID: 20132446
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-G2 Antibody (E-10) | sc-514242 | 200 µg/ml | $322.00 | |||
EF-G2 Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-531074 | 200 µg Ab; 10 µg BP | $361.00 | |||
EF-G2 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-524659 | 200 µg Ab, 40 µg BP | $361.00 | |||
EF-G2 Antibody (E-10) AC | sc-514242 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EF-G2 Antibody (E-10) HRP | sc-514242 HRP | 200 µg/ml | $322.00 | |||
EF-G2 Antibody (E-10) FITC | sc-514242 FITC | 200 µg/ml | $336.00 | |||
EF-G2 Antibody (E-10) PE | sc-514242 PE | 200 µg/ml | $349.00 | |||
EF-G2 Antibody (E-10) Alexa Fluor® 488 | sc-514242 AF488 | 200 µg/ml | $364.00 | |||
EF-G2 Antibody (E-10) Alexa Fluor® 546 | sc-514242 AF546 | 200 µg/ml | $364.00 | |||
EF-G2 Antibody (E-10) Alexa Fluor® 594 | sc-514242 AF594 | 200 µg/ml | $364.00 | |||
EF-G2 Antibody (E-10) Alexa Fluor® 647 | sc-514242 AF647 | 200 µg/ml | $364.00 | |||
EF-G2 Antibody (E-10) Alexa Fluor® 680 | sc-514242 AF680 | 200 µg/ml | $364.00 | |||
EF-G2 Antibody (E-10) Alexa Fluor® 790 | sc-514242 AF790 | 200 µg/ml | $364.00 |