EF-2 Antibody (C-9): sc-166415

EF-2 (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EF-2 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). EF-2 (C-9) antibody is available as non-conjugated anti-EF-2 antibody form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Two elongation factors (EF) EF-Tu and EF-2 participate in elongation phase during protein biosynthesis on ribosomes, with functional cycles depending on GTP binding and hydrolysis. EF-Tu (mitochondrial precursor p43) and EF-2 are multidomain GTPases essential for translation, binding to identical ribosomal sites where GTPase activities are strongly stimulated. EF-Tu delivers aminoacyl-tRNAs to translating ribosomes quickly and accurately while protecting aminoester bonds against hydrolysis until correct codon-anticodon matching occurs. EF-2 facilitates tRNA and mRNA translocation on ribosomes to expose new codons for decoding.

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EF-2 Antibody (C-9) References:

1. Possible evolution of factors involved in protein biosynthesis.  |  Nyborg, J. 1998. Acta Biochim Pol. 45: 883-94. PMID: 10397336
2. Translational regulation by modifications of the elongation factor Tu.  |  Kraal, B., et al. 1999. Folia Microbiol (Praha). 44: 131-41. PMID: 10588048
3. GTPases mechanisms and functions of translation factors on the ribosome.  |  Rodnina, MV., et al. 2000. Biol Chem. 381: 377-87. PMID: 10937868
4. Domain III of elongation factor G from Thermus thermophilus is essential for induction of GTP hydrolysis on the ribosome.  |  Martemyanov, KA. and Gudkov, AT. 2000. J Biol Chem. 275: 35820-4. PMID: 10940297
5. The molecular mechanism of eukaryotic elongation factor 2 kinase activation.  |  Tavares, CD., et al. 2014. J Biol Chem. 289: 23901-16. PMID: 25012662
6. Paradoxical Roles of Elongation Factor-2 Kinase in Stem Cell Survival.  |  Liao, Y., et al. 2016. J Biol Chem. 291: 19545-57. PMID: 27466362
7. Purification and characterization of native human elongation factor 2.  |  Flygaard, RK., et al. 2019. Protein Expr Purif. 158: 15-19. PMID: 30742898
8. Melleolides impact fungal translation via elongation factor 2.  |  Dörfer, M., et al. 2019. Org Biomol Chem. 17: 4906-4916. PMID: 31042251
9. Eukaryotic Elongation Factor 2 Kinase a Pharmacological Target to Regulate Protein Translation Dysfunction in Neurological Diseases.  |  Beretta, S., et al. 2020. Neuroscience. 445: 42-49. PMID: 32088293
10. Investigating Eukaryotic Elongation Factor 2 Kinase/Eukaryotic Translation Elongation Factor 2 Pathway Regulation and Its Role in Protein Synthesis Impairment during Disuse-Induced Skeletal Muscle Atrophy.  |  Vilchinskaya, N., et al. 2023. Am J Pathol. 193: 813-828. PMID: 36871751
11. Eukaryotic elongation factor 2 kinase inhibitor, A484954 induced diuresis via nitric oxide production in spontaneously hypertensive rats.  |  Kodama, T., et al. 2023. J Vet Med Sci. 85: 1314-1318. PMID: 37853639
12. Visualization of elongation factor G on the Escherichia coli 70S ribosome: the mechanism of translocation.  |  Agrawal, RK., et al. 1998. Proc Natl Acad Sci U S A. 95: 6134-8. PMID: 9600930

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EF-2 Antibody (C-9) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-EF-2 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EF-2 Antibody (C-9) Specifications

Alternate Product Name: EEF2

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 741-858

Epitope Target: EF-2

Epitope Species: human

Product Reactivity/Detection: EF-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for EF-2 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin