Anti-EF-2 Antibody (C-9) recommended for detection of EF-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-EF-2 Antibody (C-9): sc-166415

Anti-EF-2 Antibody (C-9) is rated 5.0 out of 5 by 5.
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Datasheets

    • Anti-EF-2 Antibody (C-9) is a mouse monoclonal IgG1 κ EF-2 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 741-858 mapping at the C-terminus of EF-2 of human origin
    • Anti-EF-2 Antibody (C-9) is recommended for detection of EF-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-EF-2 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EF-2 (C-9): sc-166415.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EF-2 Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with EF-2 Antibody (C-9) (see ordering information below).
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Can EF-2 (C-9): sc-166415 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, EF-2 (C-9): sc-166415 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Ottimo anticorpo Abbiamo acquistato l'anticorpo per EF-2 il mese scorso, ed è stato testato su lisati totali di diverse linee di fibroblasti: la banda è all'altezza attesa ed anche molto intensa. Pienamente soddisfatti
Date published: 2019-01-16
Rated 5 out of 5 by from successfully This is exactly what we need. The two elongation factors EF-Tu and EF-2 are involved in the prolongation of protein biosynthesis on ribosomes, and their functional cycle is dependent on GTP binding and its hydrolysis. With this feature, I successfully cracked the test problems
Date published: 2017-02-09
Rated 5 out of 5 by from WB positive with HeLa and HL WB positive with HeLa and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-06-27
Rated 5 out of 5 by from Worked great for IF with methanol Worked great for IF with methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-06-25
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-01-12
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