EF-1 δ Antibody (A-5): sc-393731

EF-1 δ Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects EF-1 delta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EF-1 δ (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EF-1 delta protein, also known as EEF1D, is a crucial 281 amino acid subunit of the elongation factor-1 complex, which is essential for protein synthesis as EF-1 delta facilitates the delivery of aminoacyl-tRNAs to the ribosome, thereby elongating mRNA. This process is vital for maintaining cellular function and growth, as EF-1 delta directly impacts the efficiency of protein production. Additionally, EF-1 delta functions as a guanine nucleotide exchange factor, promoting the exchange of guanosine diphosphate for guanosine triphosphate on EF-1 α, which is critical for the regulation of translation. Notably, EF-1 delta has been implicated in interactions with HIV-1 Tat, suggesting a role in the modulation of host-cell mRNA transcription. EF-1 delta overexpression has been linked to oesophageal carcinoma and may negatively influence the prognosis of medulloblastomas, highlighting EF-1 delta′s importance in the regulation of the cell cycle and transcriptional elongation. Furthermore, multiple isoforms of EF-1 delta arise from alternative splicing, which may contribute to EF-1 delta′s diverse functional roles in different cellular contexts.

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EF-1 δ Antibody (A-5) References:

1. Conserved protein kinases encoded by herpesviruses and cellular protein kinase cdc2 target the same phosphorylation site in eukaryotic elongation factor 1delta.  |  Kawaguchi, Y., et al. 2003. J Virol. 77: 2359-68. PMID: 12551973
2. Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1A.  |  Cans, C., et al. 2003. Proc Natl Acad Sci U S A. 100: 13892-7. PMID: 14623968
3. Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation.  |  Brandenberger, R., et al. 2004. Nat Biotechnol. 22: 707-16. PMID: 15146197
4. The molecular mechanics of eukaryotic translation.  |  Kapp, LD. and Lorsch, JR. 2004. Annu Rev Biochem. 73: 657-704. PMID: 15189156
5. Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma.  |  Ogawa, K., et al. 2004. Br J Cancer. 91: 282-6. PMID: 15199388
6. Phosphoproteomic analysis of the human pituitary.  |  Beranova-Giorgianni, S., et al. 2006. Pituitary. 9: 109-20. PMID: 16807684
7. Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8.  |  De Bortoli, M., et al. 2006. BMC Cancer. 6: 223. PMID: 16968546
8. BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells.  |  Yang, S., et al. 2007. BMC Cancer. 7: 211. PMID: 17997862
9. A study on the fundamental factors determining the efficacy of siRNAs with high C/G contents.  |  Liao, JY., et al. 2008. Cell Mol Biol Lett. 13: 283-302. PMID: 18197393
10. Alternative expression and sequence of human elongation factor-1 delta during malignant transformation of human bronchial epithelial cells induced by cadmium chloride.  |  Lei, YX., et al. 2010. Biomed Environ Sci. 23: 151-7. PMID: 20514991
11. GDP as a regulator of phosphorylation of elongation factor 1 by casein kinase II.  |  Palen, E., et al. 1994. Biochemistry. 33: 8515-20. PMID: 8031785
12. The human leucine zipper-containing guanine-nucleotide exchange protein elongation factor-1 delta.  |  Sanders, J., et al. 1993. Biochim Biophys Acta. 1174: 87-90. PMID: 8334168

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EF-1 δ Antibody (A-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-EF-1 δ Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EF-1 δ Antibody (A-5) Specifications

Alternate Product Name: EF-1 δ

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-153

Epitope Target: EF-1 δ

Epitope Species: human

Product Reactivity/Detection: EF-1 δ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for EF-1 δ Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin