EDG-1/S1P1/S1PR1 Antibody (A-6): sc-48356

EDG-1 Antibody (A-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects EDG-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EDG-1 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EDG-1, also known as S1PR1 or S1P1, is a member of the endothelial differentiation gene (EDG) family of G protein-coupled receptors, which play a crucial role in mediating the effects of lysophospholipid signaling molecules such as sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA). EDG-1 is primarily located on the cell surface and is involved in various cellular processes, including cell survival, growth, migration, and differentiation, by coupling to Gi proteins to activate downstream signaling pathways like Akt. This is particularly important in glioma cells, where EDG-1 expression is associated with tumor progression and survival. EDG-1′s ability to interact with multiple G proteins influences a wide range of physiological responses, making EDG-1 a significant target for research in cancer biology and therapeutic development.

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EDG-1/S1P1/S1PR1 Antibody (A-6) References:

1. Differential pharmacological properties and signal transduction of the sphingosine 1-phosphate receptors EDG-1, EDG-3, and EDG-5.  |  Ancellin, N. and Hla, T. 1999. J Biol Chem. 274: 18997-9002. PMID: 10383399
2. A subfamily of G protein-coupled cellular receptors for lysophospholipids and lysosphingolipids.  |  Goetzl, EJ. and An, S. 1999. Adv Exp Med Biol. 469: 259-64. PMID: 10667339
3. Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6.  |  Van Brocklyn, JR., et al. 2000. Blood. 95: 2624-9. PMID: 10753843
4. Sphingosine-1-phosphate signaling via the EDG-1 family of G-protein-coupled receptors.  |  Hla, T., et al. 2000. Ann N Y Acad Sci. 905: 16-24. PMID: 10818438
5. Sphingosine 1-phosphate: a ligand for the EDG-1 family of G-protein-coupled receptors.  |  Spiegel, S. 2000. Ann N Y Acad Sci. 905: 54-60. PMID: 10818441
6. Sphingosine 1-phosphate signalling in mammalian cells.  |  Pyne, S. and Pyne, NJ. 2000. Biochem J. 349: 385-402. PMID: 10880336
7. Differential roles of Edg-1 and Edg-5, sphingosine 1-phosphate receptors, in the signaling pathways in C6 glioma cells.  |  Sato, K., et al. 2000. Brain Res Mol Brain Res. 85: 151-60. PMID: 11146117
8. Lysophosphatidic acid receptor-selective effects on Jurkat T cell migration through a Matrigel model basement membrane.  |  Zheng, Y., et al. 2001. J Immunol. 166: 2317-22. PMID: 11160288
9. Edg2 receptor distribution in adult rat brain.  |  Handford, EJ., et al. 2001. Neuroreport. 12: 757-60. PMID: 11277579
10. Sphingosine 1-phosphate activates Akt, nitric oxide production, and chemotaxis through a Gi protein/phosphoinositide 3-kinase pathway in endothelial cells.  |  Morales-Ruiz, M., et al. 2001. J Biol Chem. 276: 19672-7. PMID: 11278592
11. Roles for N-glycosylation in the dynamics of Edg-1/S1P1 in sphingosine 1-phosphate-stimulated cells.  |  Kohno, T. and Igarashi, Y. 2004. Glycoconj J. 21: 497-501. PMID: 15750791

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EDG-1/S1P1/S1PR1 Antibody (A-6) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-EDG-1/S1P1/S1PR1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EDG-1/S1P1/S1PR1 Antibody (A-6) Specifications

Alternate Product Name: EDG1

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 322-381

Epitope Target: EDG-1

Epitope Species: human

Product Reactivity/Detection: EDG-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for EDG-1/S1P1/S1PR1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin