EDEM2 Antibody (JD-32): sc-130460

EDEM2 Antibody (JD-32) is a mouse monoclonal IgG1 kappa light chain antibody that detects EDEM2 protein of human origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-EDEM2 antibody (JD-32) is available as a non-conjugated format. EDEM2, or ER degradation-enhancing alpha-mannosidase-like 2, plays a crucial role in the endoplasmic reticulum (ER) quality control system, specifically in the process known as ER-associated degradation (ERAD). This pathway is essential for maintaining cellular homeostasis by ensuring that misfolded or improperly assembled proteins are efficiently identified and targeted for degradation. EDEM2 is a 578 amino acid secreted protein that works in concert with other EDEM family members, such as EDEM and EDEM3, to facilitate the retrotranslocation of terminally misfolded proteins from the ER to the cytoplasm, where these proteins are subsequently degraded by proteasomes. EDEM2 contains a mannosidase homology domain, an N-terminal cleavable signal sequence, and a C-terminal extension that is vital for ER retention and overall functionality. Human EDEM2 shares 93% sequence identity with its mouse counterpart, indicating a conserved role across species, and two EDEM2 isoforms arise from alternative splicing events, further highlighting the complexity and regulatory mechanisms governing protein quality control in the ER.

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EDEM2 Antibody (JD-32) References:

1. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.  |  Clark, HF., et al. 2003. Genome Res. 13: 2265-70. PMID: 12975309
2. Human EDEM2, a novel homolog of family 47 glycosidases, is involved in ER-associated degradation of glycoproteins.  |  Mast, SW., et al. 2005. Glycobiology. 15: 421-36. PMID: 15537790
3. A novel stress-induced EDEM variant regulating endoplasmic reticulum-associated glycoprotein degradation.  |  Olivari, S., et al. 2005. J Biol Chem. 280: 2424-8. PMID: 15579471
4. Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation.  |  Oda, Y., et al. 2006. J Cell Biol. 172: 383-93. PMID: 16449189
5. Glycoprotein folding and the role of EDEM1, EDEM2 and EDEM3 in degradation of folding-defective glycoproteins.  |  Olivari, S. and Molinari, M. 2007. FEBS Lett. 581: 3658-64. PMID: 17499246

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EDEM2 Antibody (JD-32) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-EDEM2 Antibody (JD-32) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EDEM2 Antibody (JD-32) Specifications

Alternate Product Name: EDEM2

Product Clone Name: JD-32

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 479-579

Epitope Target: EDEM2

Epitope Species: human

Product Reactivity/Detection: EDEM2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for EDEM2 Antibody (JD-32): N/A