EDEM Antibody (D-1): sc-377394

EDEM Antibody (D-1) is a mouse monoclonal IgM antibody that detects EDEM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EDEM (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EDEM protein plays a crucial role in the endoplasmic reticulum-associated degradation (ERAD) pathway, which is essential for maintaining cellular homeostasis by ensuring that misfolded or improperly assembled proteins are efficiently identified and targeted for degradation. Specifically, EDEM functions by recognizing misfolded glycoproteins and facilitating their extraction from the calnexin cycle, thereby promoting their retrotranslocation to the cytoplasm for proteasomal degradation. This process is vital for preventing the accumulation of dysfunctional proteins that could lead to cellular stress and disease. The human EDEM gene is located on chromosome 3p, highlighting its evolutionary conservation and importance across species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EDEM Antibody (D-1) References:

1. A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation.  |  Hosokawa, N., et al. 2001. EMBO Rep. 2: 415-22. PMID: 11375934
2. A time-dependent phase shift in the mammalian unfolded protein response.  |  Yoshida, H., et al. 2003. Dev Cell. 4: 265-71. PMID: 12586069
3. EDEM as an acceptor of terminally misfolded glycoproteins released from calnexin.  |  Oda, Y., et al. 2003. Science. 299: 1394-7. PMID: 12610305
4. Hepatitis C virus suppresses the IRE1-XBP1 pathway of the unfolded protein response.  |  Tardif, KD., et al. 2004. J Biol Chem. 279: 17158-64. PMID: 14960590
5. Candidate genes for recessive non-syndromic mental retardation on chromosome 3p (MRT2A).  |  Higgins, JJ., et al. 2004. Clin Genet. 65: 496-500. PMID: 15151510
6. EDEM contributes to maintenance of protein folding efficiency and secretory capacity.  |  Eriksson, KK., et al. 2004. J Biol Chem. 279: 44600-5. PMID: 15308654
7. Protein-tyrosine phosphatase 1B potentiates IRE1 signaling during endoplasmic reticulum stress.  |  Gu, F., et al. 2004. J Biol Chem. 279: 49689-93. PMID: 15465829
8. A novel stress-induced EDEM variant regulating endoplasmic reticulum-associated glycoprotein degradation.  |  Olivari, S., et al. 2005. J Biol Chem. 280: 2424-8. PMID: 15579471
9. EDEM3, a soluble EDEM homolog, enhances glycoprotein endoplasmic reticulum-associated degradation and mannose trimming.  |  Hirao, K., et al. 2006. J Biol Chem. 281: 9650-8. PMID: 16431915
10. EDEM is involved in retrotranslocation of ricin from the endoplasmic reticulum to the cytosol.  |  Slominska-Wojewodzka, M., et al. 2006. Mol Biol Cell. 17: 1664-75. PMID: 16452630
11. Profiling Optimal Conditions for Capturing EDEM Proteins Complexes in Melanoma Using Mass Spectrometry.  |  Munteanu, CVA., et al. 2019. Adv Exp Med Biol. 1140: 155-167. PMID: 31347047
12. PCV2 and PRV Coinfection Induces Endoplasmic Reticulum Stress via PERK-eIF2α-ATF4-CHOP and IRE1-XBP1-EDEM Pathways.  |  Chen, S., et al. 2022. Int J Mol Sci. 23: PMID: 35562870

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EDEM Antibody (D-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-EDEM Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EDEM Antibody (D-1) Specifications

Alternate Product Name: EDEM1

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 623-651

Epitope Target: EDEM

Epitope Species: human

Product Reactivity/Detection: EDEM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for EDEM Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin