Ect2 Antibody (G-4): sc-514750

Ect2 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ect2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ect2 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ect2 protein plays a crucial role in regulating the actin cytoskeleton, which is essential for various cellular processes such as cell division, migration, and shape maintenance. Specifically, Ect2 acts as a guanine nucleotide exchange factor (GEF) for RhoA, a member of the Rho family of GTPases, which are pivotal in controlling the dynamics of the cytoskeleton. By facilitating the exchange of GDP for GTP on RhoA, Ect2 promotes the activation of downstream signaling pathways that lead to actin polymerization and the formation of contractile structures, thereby influencing cell motility and morphology. The significance of Ect2 in these processes is highlighted by its involvement in cancer progression, where irregular regulation of the actin cytoskeleton can contribute to increased cell invasiveness and metastasis. Ect2 monoclonal antibody (G-4) serves as a valuable tool for researchers studying the molecular mechanisms underlying cell behavior and the role of GTPases in health and disease.

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Ect2 Antibody (G-4) References:

1. Development of a highly efficient expression cDNA cloning system: application to oncogene isolation.  |  Miki, T., et al. 1991. Proc Natl Acad Sci U S A. 88: 5167-71. PMID: 2052597
2. A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, CDC24, and the human breakpoint cluster gene, bcr.  |  Ron, D., et al. 1991. New Biol. 3: 372-9. PMID: 2065022
3. Oncogenic Ect2 signaling regulates rRNA synthesis in NSCLC.  |  Justilien, V., et al. 2019. Small GTPases. 10: 388-394. PMID: 28657426
4. Increased expression of ECT2 predicts the poor prognosis of breast cancer patients.  |  Yi, M., et al. 2022. Exp Hematol Oncol. 11: 107. PMID: 36572949
5. GINS2 regulates temozolomide chemosensitivity via the EGR1/ECT2 axis in gliomas.  |  He, H., et al. 2024. Cell Death Dis. 15: 205. PMID: 38467631
6. Elevated expression of ECT2 as a diagnostic marker and prognostic indicator in endometrial cancer.  |  Wu, XG., et al. 2024. Gene. 927: 148756. PMID: 38977110
7. GTPase cascades choreographing cellular behavior: movement, morphogenesis, and more.  |  Chant, J. and Stowers, L. 1995. Cell. 81: 1-4. PMID: 7720065
8. A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways.  |  Horii, Y., et al. 1994. EMBO J. 13: 4776-86. PMID: 7957046
9. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
10. Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the dbl oncogene product.  |  Hart, MJ., et al. 1994. J Biol Chem. 269: 62-5. PMID: 8276860
11. Oncogene ect2 is related to regulators of small GTP-binding proteins.  |  Miki, T., et al. 1993. Nature. 362: 462-5. PMID: 8464478
12. A putative modular domain present in diverse signaling proteins.  |  Mayer, BJ., et al. 1993. Cell. 73: 629-30. PMID: 8500161

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ect2 Antibody (G-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Ect2 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ect2 Antibody (G-4) Specifications

Alternate Product Name: ECT2

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 584-883

Epitope Target: Ect2

Epitope Species: human

Product Reactivity/Detection: Ect2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Ect2 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin