ECM1 Antibody (F-1): sc-365335

ECM1 Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects ECM1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ECM1 antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ECM1, or extracellular matrix protein 1, is a secreted glycoprotein that plays a crucial role in maintaining the structural integrity and function of the skin. ECM1 is predominantly localized in the extracellular matrix, where ECM1 interacts with several key components, such as Perlecan, Fibulin, and matrix metalloproteinase-9 (MMP-9), thereby contributing to the organization of the dermis. ECM1 enhances the binding of Collagen Type IV to Laminin 332, highlighting ECM1′s importance in the interstitial dermis and the dermal-epidermal junction. Mutations in the ECM1 gene are linked to the autosomal recessive disorder lipoid proteinosis (LiP), which is characterized by dermal hyalinization and basement membrane reduplication, leading to skin and mucosal thickening in affected individuals. ECM1 has four splice variants (ECM1a-ECM1d), each potentially contributing to diverse biological functions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ECM1 Antibody (F-1) References:

1. ECM1 promotes the Warburg effect through EGF-mediated activation of PKM2.  |  Lee, KM., et al. 2015. Cell Signal. 27: 228-35. PMID: 25446258
2. ECM1 regulates tumor metastasis and Csc-like property through stabilization of β-catenin.  |  Lee, KM., et al. 2015. Oncogene. 34: 6055-65. PMID: 25746001
3. ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition.  |  Chen, H., et al. 2016. World J Surg Oncol. 14: 195. PMID: 27460906
4. ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.  |  Fan, W., et al. 2019. Gastroenterology. 157: 1352-1367.e13. PMID: 31362006
5. ECM1 is an essential factor for the determination of M1 macrophage polarization in IBD in response to LPS stimulation.  |  Zhang, Y., et al. 2020. Proc Natl Acad Sci U S A. 117: 3083-3092. PMID: 31980528
6. ECM1 promotes migration and invasion in endometriosis.  |  Zhang, C., et al. 2024. Reprod Biol. 24: 100826. PMID: 37992590
7. Serum ECM1 is a promising biomarker for staging and monitoring fibrosis in patients with chronic hepatitis B.  |  Liu, L., et al. 2025. Sci China Life Sci. 68: 431-440. PMID: 39348048
8. ECM1 attenuates hepatic fibrosis by interfering with mediators of latent TGF-β1 activation.  |  Link, F., et al. 2025. Gut. 74: 424-439. PMID: 39448254
9. Mechanistic study of celastrol-mediated inhibition of proinflammatory activation of macrophages in IgA nephropathy via down-regulating ECM1.  |  Zhao, J., et al. 2024. Int J Biol Sci. 20: 5731-5746. PMID: 39494325
10. Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer.  |  Wang, X., et al. 2025. Adv Sci (Weinh). 12: e2407662. PMID: 39563492

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ECM1 Antibody (F-1) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-ECM1 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ECM1 Antibody (F-1) Specifications

Alternate Product Name: ECM1

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 161-220

Epitope Target: ECM1

Epitope Species: human

Product Reactivity/Detection: ECM1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for ECM1 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ECM1 antibody is ECM1 Antibody (C-12): sc-365946.