ECM1 Antibody (C-12): sc-365946

ECM1 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects ECM1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ECM1 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ECM1, or extracellular matrix protein 1, is a secreted glycoprotein that plays a crucial role in maintaining the structure and function of the skin, as ECM1 is widely expressed and primarily localizes to the extracellular matrix. ECM1 is known to interact with several key extracellular matrix components, such as Perlecan, Fibulin, and matrix metalloproteinase-9 (MMP-9), which are essential for the structural organization of the dermis. Notably, ECM1 enhances the association of Collagen Type IV with Laminin 332, indicating ECM1′s significant role in the interstitial dermis and the dermal-epidermal junction. Mutations in the gene encoding ECM1 can lead to the autosomal recessive disorder lipoid proteinosis (LiP), characterized by dermal hyalinization and basement membrane reduplication, resulting in thickened skin and mucosae in affected individuals. Additionally, there are four splice variants of ECM1, designated ECM1a through ECM1d, which may have distinct functional roles in tissue organization and repair.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ECM1 Antibody (C-12) References:

1. Extracellular matrix protein 1 interacts with the domain III of fibulin-1C and 1D variants through its central tandem repeat 2.  |  Fujimoto, N., et al. 2005. Biochem Biophys Res Commun. 333: 1327-33. PMID: 15990087
2. ECM1 and TMPRSS4 are diagnostic markers of malignant thyroid neoplasms and improve the accuracy of fine needle aspiration biopsy.  |  Kebebew, E., et al. 2005. Ann Surg. 242: 353-61; discussion 361-3. PMID: 16135921
3. A novel mutation of the extracellular matrix protein 1 gene (ECM1) in a patient with lipoid proteinosis (Urbach-Wiethe disease) from Sicily.  |  Lupo, I., et al. 2005. Br J Dermatol. 153: 1019-22. PMID: 16225617
4. A novel splice-site mutation in ECM-1 gene in a consanguineous family with lipoid proteinosis.  |  Horev, L., et al. 2005. Exp Dermatol. 14: 891-7. PMID: 16274456
5. Expression of extracellular matrix protein 1 (ECM1) in human skin is decreased by age and increased upon ultraviolet exposure.  |  Sander, CS., et al. 2006. Br J Dermatol. 154: 218-24. PMID: 16433788
6. Extracellular matrix protein 1 inhibits the activity of matrix metalloproteinase 9 through high-affinity protein/protein interactions.  |  Fujimoto, N., et al. 2006. Exp Dermatol. 15: 300-7. PMID: 16512877
7. Functional redundancy of extracellular matrix protein 1 in epidermal differentiation.  |  Sercu, S., et al. 2007. Br J Dermatol. 157: 771-5. PMID: 17711528
8. The molecular basis of lipoid proteinosis: mutations in extracellular matrix protein 1.  |  Chan, I., et al. 2007. Exp Dermatol. 16: 881-90. PMID: 17927570
9. Interaction of extracellular matrix protein 1 with extracellular matrix components: ECM1 is a basement membrane protein of the skin.  |  Sercu, S., et al. 2008. J Invest Dermatol. 128: 1397-408. PMID: 18200062
10. Novel role of extracellular matrix protein 1 (ECM1) in cardiac aging and myocardial infarction.  |  Hardy, SA., et al. 2019. PLoS One. 14: e0212230. PMID: 30789914
11. Wentilactone A Reverses the NF-κB/ECM1 Signaling-Induced Cisplatin Resistance through Inhibition of IKK/IκB in Ovarian Cancer Cells.  |  Lv, C., et al. 2022. Nutrients. 14: PMID: 36145166
12. TET2-mediated ECM1 hypomethylation promotes the neovascularization in active proliferative diabetic retinopathy.  |  Cai, C., et al. 2024. Clin Epigenetics. 16: 6. PMID: 38172938

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ECM1 Antibody (C-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ECM1 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ECM1 Antibody (C-12) Specifications

Alternate Product Name: ECM1

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 43-77

Epitope Target: ECM1

Epitope Species: human

Product Reactivity/Detection: ECM1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ECM1 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ECM1 antibody is ECM1 Antibody (F-1): sc-365335.