EBP Antibody (A-9): sc-374267
- EBP Antibody (A-9) is a mouse monoclonal IgG1 κ EBP antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-25 at the N-terminus of EBP of human origin
- EBP Antibody (A-9) is recommended for detection of EBP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-EBP Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EBP Antibody (A-9) for WB and IHC(P) applications. These reagents are now offered in bundles with EBP Antibody (A-9) (see ordering information below).
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SEE ALSO...
EBP Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EBP (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EBP protein, also known as emopamil-binding protein, CPX, CHO2, CPXD, or CDPX2, is a 230 amino acid multi-pass membrane protein primarily located in the endoplasmic reticulum (ER). This localization is crucial as EBP plays a significant role in steroid biosynthesis, specifically catalyzing the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers, which is essential for maintaining cholesterol homeostasis and facilitating drug transport within the ER. Importantly, defects in the gene encoding EBP can lead to chondrodysplasia punctata X-linked dominant type 2 (CDPX2), a disorder characterized by defective cholesterol biosynthesis that manifests as punctiform calcification of bones, linear ichthyosis, cataracts, and short stature. Anti-EBP antibody (A-9) effectively detects this protein across multiple applications, making EBP (A-9) monoclonal antibody a valuable tool for researchers studying cholesterol metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EBP Antibody (A-9) References:
- Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com. | Derry, JM., et al. 1999. Nat Genet. 22: 286-90. PMID: 10391218
- Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome. | Braverman, N., et al. 1999. Nat Genet. 22: 291-4. PMID: 10391219
- The Conradi-Hünermann-Happle syndrome (CDPX2) and emopamil binding protein: novel mutations, and somatic and gonadal mosaicism. | Has, C., et al. 2000. Hum Mol Genet. 9: 1951-5. PMID: 10942423
- Identification of a novel mutation in 3beta-hydroxysteroid-Delta8-Delta7-isomerase in a case of Conradi-Hünermann-Happle syndrome. | Becker, K., et al. 2001. Exp Dermatol. 10: 286-9. PMID: 11493318
- Cloning of an emopamil-binding protein (EBP)-like protein that lacks sterol delta8-delta7 isomerase activity. | Moebius, FF., et al. 2003. Biochem J. 374: 229-37. PMID: 12760743
- Novel EBP gene mutations in Conradi-Hünermann-Happle syndrome. | Steijlen, PM., et al. 2007. Br J Dermatol. 157: 1225-9. PMID: 17949453
- Two novel EBP mutations in Conradi-Hünermann-Happle syndrome. | Ausavarat, S., et al. 2008. Eur J Dermatol. 18: 391-3. PMID: 18573709
- Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations. | Lu, Y., et al. 2008. J Lipid Res. 49: 2582-9. PMID: 18660489
- Phenylalkylamine Ca2+ antagonist binding protein. Molecular cloning, tissue distribution, and heterologous expression. | Hanner, M., et al. 1995. J Biol Chem. 270: 7551-7. PMID: 7706302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EBP Antibody (A-9) | sc-374267 | 200 µg/ml | $316.00 | |||
EBP Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-529705 | 200 µg Ab; 10 µg BP | $354.00 | |||
EBP Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-522680 | 200 µg Ab, 40 µg BP | $354.00 | |||
EBP Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-543642 | 200 µg Ab; 20 µg BP | $354.00 | |||
EBP Antibody (A-9) AC | sc-374267 AC | 500 µg/ml, 25% agarose | $416.00 | |||
EBP Antibody (A-9) HRP | sc-374267 HRP | 200 µg/ml | $316.00 | |||
EBP Antibody (A-9) FITC | sc-374267 FITC | 200 µg/ml | $330.00 | |||
EBP Antibody (A-9) PE | sc-374267 PE | 200 µg/ml | $343.00 | |||
EBP Antibody (A-9) Alexa Fluor® 488 | sc-374267 AF488 | 200 µg/ml | $357.00 | |||
EBP Antibody (A-9) Alexa Fluor® 546 | sc-374267 AF546 | 200 µg/ml | $357.00 | |||
EBP Antibody (A-9) Alexa Fluor® 594 | sc-374267 AF594 | 200 µg/ml | $357.00 | |||
EBP Antibody (A-9) Alexa Fluor® 647 | sc-374267 AF647 | 200 µg/ml | $357.00 | |||
EBP Antibody (A-9) Alexa Fluor® 680 | sc-374267 AF680 | 200 µg/ml | $357.00 | |||
EBP Antibody (A-9) Alexa Fluor® 790 | sc-374267 AF790 | 200 µg/ml | $357.00 | |||
EBP (A-9) Neutralizing Peptide | sc-374267 P | 100 µg/0.5 ml | $68.00 |