Ebi3 Antibody (F-7): sc-515323

Ebi3 Antibody (F-7) is a mouse monoclonal IgM antibody that detects Epstein-Barr virus-induced gene 3 (Ebi3) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ebi3 is a crucial component of the interleukin-12 family, functioning as a homolog to the IL-12 p40 subunit and forming heterodimers with IL-12 p35 or its homolog p28 to produce the cytokine IL-27. This cytokine plays a significant role in the immune response by promoting the rapid clonal expansion of naive CD4+ T cells, which is essential for initiating effective adaptive immunity. The regulation of Ebi3 expression is influenced by interferon-beta, which modulates the levels of various IL-12 family members in activated human dendritic cells, highlighting its importance in immune signaling. Notably, Ebi3 is highly expressed in Hodgkin and Reed-Sternberg cells, suggesting its potential role in evading anti-tumor immune responses. The human Ebi3 gene is located on chromosome 19p13.3 and encodes a secreted glycoprotein predominantly found in the spleen, tonsils, and at elevated levels in the full-term placenta, making Ebi3 a valuable target for research in immunology and cancer biology. Anti-Ebi3 antibody (F-7) is an essential tool for scientists investigating the complex interactions within the immune system and the mechanisms of immune evasion in tumors.

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Ebi3 Antibody (F-7) References:

1. Analysis of interleukin-27 (EBI3/p28) expression in Epstein-Barr virus- and human T-cell leukemia virus type 1-associated lymphomas: heterogeneous expression of EBI3 subunit by tumoral cells.  |  Larousserie, F., et al. 2005. Am J Pathol. 166: 1217-28. PMID: 15793300
2. EBI3 deficiency leads to diminished T helper type 1 and increased T helper type 2 mediated airway inflammation.  |  Dokmeci, E., et al. 2011. Immunology. 132: 559-66. PMID: 21255010
3. Decreased expression of EBI3 and Foxp3 in CD4+CD25+ regulatory T cells in murine experimental allergic rhinitis.  |  Ouyang, Y., et al. 2011. ORL J Otorhinolaryngol Relat Spec. 73: 313-20. PMID: 21986066
4. Epstein-Barr virus-induced gene 3 (EBI3) polymorphisms and expression are associated with susceptibility to pulmonary tuberculosis.  |  Zheng, R., et al. 2015. Tuberculosis (Edinb). 95: 497-504. PMID: 25937126
5. Higher decidual EBI3 and HLA-G mRNA expression in preeclampsia: Cause or consequence of preeclampsia.  |  Prins, JR., et al. 2016. Hum Immunol. 77: 68-70. PMID: 26472010
6. Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway.  |  Zhang, SA., et al. 2017. Tumour Biol. 39: 1010428317692237. PMID: 28351328
7. Significant reduction of peripheral blood interleukin-35 and CD4+EBI3+ T cells, which are negatively correlated with an increase in the plasma IL-17 and cTnI level, in viral myocarditis patients.  |  Ouyang, H., et al. 2017. Cent Eur J Immunol. 42: 91-96. PMID: 28680336
8. Epigenetic and parasitological parameters are modulated in EBi3-/- mice infected with Schistosoma mansoni.  |  Mota, EA., et al. 2020. PLoS Negl Trop Dis. 14: e0008080. PMID: 32078636
9. Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes.  |  Tachibana, K., et al. 2021. Int J Mol Sci. 22: PMID: 34884474
10. Evaluation of IL-23p19/Ebi3 (IL-39) gingival crevicular fluid levels in periodontal health, gingivitis, and periodontitis.  |  Sari, A., et al. 2022. Clin Oral Investig. 26: 7209-7218. PMID: 35986791

The preferred Ebi3 antibody is Ebi3 Antibody (G-4): sc-166158.