EB1 Antibody (F-7): sc-374474

EB1 Antibody (F-7) is a mouse monoclonal IgA antibody that detects EB1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). EB1, also known as microtubule-associated protein RP/EB family member 1 (MAPRE1) or APC-binding protein EB1, plays a crucial role in the regulation of microtubule dynamics, which is essential for various cellular processes such as cell division, intracellular transport, and maintaining cell shape. EB1 (F-7) antibody interacts with the plus ends of growing microtubules, facilitating their stabilization and promoting the formation of microtubule structures necessary for effective cellular function. Dysregulation of EB1 has been implicated in tumorigenesis, particularly in colorectal cancers, where EB1 may influence the transition from normal colonic epithelium to adenomatous polyps and invasive cancer. Notably, EB1 binds to the carboxy terminal region of the adenomatous polyposis coli (APC) tumor suppressor protein, suggesting a significant role in modulating APC′s function in cancer suppression. Overexpression of EB1 can disrupt this interaction, potentially leading to aberrant activation of the β-catenin/TCF signaling pathway, which is a critical factor in the development of various cancers, including human esophageal squamous cell carcinoma.

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EB1 Antibody (F-7) References:

1. Regulation of EB1/3 proteins by classical MAPs in neurons.  |  Sayas, CL. and Avila, J. 2014. Bioarchitecture. 4: 1-5. PMID: 24452057
2. Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk.  |  Wei, J., et al. 2017. FEBS Lett. 591: 2379-2393. PMID: 28726242
3. EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.  |  Bouguenina, H., et al. 2017. Proc Natl Acad Sci U S A. 114: E10687-E10696. PMID: 29162697
4. PTPN22 interacts with EB1 to regulate T-cell receptor signaling.  |  Zhang, X., et al. 2020. FASEB J. 34: 8959-8974. PMID: 32469452
5. EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte Meiotic Maturation in Mice.  |  Zhou, D., et al. 2021. Microsc Microanal. 27: 385-391. PMID: 33413706
6. EB1 Restricts Breast Cancer Cell Invadopodia Formation and Matrix Proteolysis via FAK.  |  Chanez, B., et al. 2021. Cells. 10: PMID: 33668531
7. Optogenetic EB1 inactivation shortens metaphase spindles by disrupting cortical force-producing interactions with astral microtubules.  |  Dema, A., et al. 2022. Curr Biol. 32: 1197-1205.e4. PMID: 35090591
8. Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant.  |  Dema, A., et al. 2023. Elife. 12: PMID: 36715499
9. Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages.  |  Zeeshan, M., et al. 2023. Nat Commun. 14: 5652. PMID: 37704606
10. Rapid binding to protofilament edge sites facilitates tip tracking of EB1 at growing microtubule plus-ends.  |  Gonzalez, SJ., et al. 2024. Elife. 13: PMID: 38385657

The preferred EB1 antibody is EB1 Antibody (1A11/4): sc-47704.