EB1 Antibody (1A11/4): sc-47704
- EB1 Antibody (1A11/4) is a mouse monoclonal IgG2b κ EB1 antibody, cited in 23 publications, provided at 200 µg/ml
- raised against EB1 δC1 mutant
- EB1 Antibody (1A11/4) is recommended for detection of EB1 of human, canine and monkey origin by WB, IP, IF and IHC(P)
- Anti-EB1 Antibody (1A11/4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EB1 Antibody (1A11/4) for WB and IHC(P) applications. These reagents are now offered in bundles with EB1 Antibody (1A11/4) (see ordering information below).
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EB1 Antibody (1A11/4) is a mouse monoclonal IgG2b kappa light chain antibody that detects EB1 protein of human, canine, and monkey origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). EB1 (1A11/4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EB1, also known as microtubule-associated protein RP/EB family member 1 (MAPRE1) or APC-binding protein EB1, plays a crucial role in microtubule dynamics and is essential for proper cell division and intracellular transport. MAPRE1 function is particularly important in cancer biology, as EB1 regulates the adenomatous polyposis coli (APC) tumor suppressor protein, which prevents colorectal cancer. Mutations in APC can lead to loss of regulatory functions, resulting in uncontrolled cell proliferation and tumorigenesis. Through binding to APC′s carboxy terminal region, EB1 helps mediate tumor-suppressive effects, and dysregulation of MAPRE1 expression has been implicated in various cancers, including esophageal squamous cell carcinoma. EB1′s influence on microtubule stability and interactions with other proteins in signaling pathways further underscore MAPRE1′s significance in both normal cellular processes and cancer progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EB1 Antibody (1A11/4) References:
- EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability. | Tirnauer, JS. and Bierer, BE. 2000. J Cell Biol. 149: 761-6. PMID: 10811817
- Molecular analysis of APC mutations in familial adenomatous polyposis and sporadic colon carcinomas. | Cottrell, S., et al. 1992. Lancet. 340: 626-30. PMID: 1355210
- Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end. | Slep, KC., et al. 2005. J Cell Biol. 168: 587-98. PMID: 15699215
- CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites. | Bieling, P., et al. 2008. J Cell Biol. 183: 1223-33. PMID: 19103809
- Microtubule plus-end tracking by CLIP-170 requires EB1. | Dixit, R., et al. 2009. Proc Natl Acad Sci U S A. 106: 492-7. PMID: 19126680
- Microtubule plus-end binding protein EB1 is necessary for muscle cell differentiation, elongation and fusion. | Zhang, T., et al. 2009. J Cell Sci. 122: 1401-9. PMID: 19366726
- Migration and actin protrusion in melanoma cells are regulated by EB1 protein. | Schober, JM., et al. 2009. Cancer Lett. 284: 30-6. PMID: 19427113
- EB1 Directly Regulates APC-Mediated Actin Nucleation. | Juanes, MA., et al. 2020. Curr Biol. 30: 4763-4772.e8. PMID: 33007249
- Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis. | Song, X., et al. 2021. Nat Chem Biol. 17: 1314-1323. PMID: 34608293
- Phase separation of EB1 guides microtubule plus-end dynamics. | Song, X., et al. 2023. Nat Cell Biol. 25: 79-91. PMID: 36536176
- APC binds to the novel protein EB1. | Su, LK., et al. 1995. Cancer Res. 55: 2972-7. PMID: 7606712
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EB1 Antibody (1A11/4) | sc-47704 | 200 µg/ml | $322.00 | |||
EB1 Antibody (1A11/4): m-IgG Fc BP-HRP Bundle | sc-528509 | 200 µg Ab; 10 µg BP | $361.00 | |||
EB1 Antibody (1A11/4): m-IgGκ BP-HRP Bundle | sc-520903 | 200 µg Ab, 40 µg BP | $361.00 | |||
EB1 Antibody (1A11/4): m-IgG2b BP-HRP Bundle | sc-550048 | 200 µg Ab; 10 µg BP | $361.00 | |||
EB1 Antibody (1A11/4) AC | sc-47704 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EB1 Antibody (1A11/4) HRP | sc-47704 HRP | 200 µg/ml | $322.00 | |||
EB1 Antibody (1A11/4) FITC | sc-47704 FITC | 200 µg/ml | $336.00 | |||
EB1 Antibody (1A11/4) PE | sc-47704 PE | 200 µg/ml | $349.00 | |||
EB1 Antibody (1A11/4) Alexa Fluor® 488 | sc-47704 AF488 | 200 µg/ml | $364.00 | |||
EB1 Antibody (1A11/4) Alexa Fluor® 546 | sc-47704 AF546 | 200 µg/ml | $364.00 | |||
EB1 Antibody (1A11/4) Alexa Fluor® 594 | sc-47704 AF594 | 200 µg/ml | $364.00 | |||
EB1 Antibody (1A11/4) Alexa Fluor® 647 | sc-47704 AF647 | 200 µg/ml | $364.00 | |||
EB1 Antibody (1A11/4) Alexa Fluor® 680 | sc-47704 AF680 | 200 µg/ml | $364.00 | |||
EB1 Antibody (1A11/4) Alexa Fluor® 790 | sc-47704 AF790 | 200 µg/ml | $364.00 |