EAG Antibody (C-9): sc-377236
- EAG Antibody (C-9) is a mouse monoclonal IgG1 κ EAG antibody provided at 200 µg/ml
- raised against amino acids 215-429 of EAG of Streptococcus equi subsp. equi origin
- EAG Antibody (C-9) is recommended for detection of of S. equi origin by WB, IP, IF and ELISA
- Anti-EAG Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EAG Antibody (C-9) for WB applications. These reagents are now offered in bundles with EAG Antibody (C-9) (see ordering information below).
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SEE ALSO...
EAG Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EAG protein of S. equi origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EAG (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EAG protein, also known as Ig, alpha2-macroglobulin and albumin binding protein Eag, is a 429 amino acid protein that plays a crucial role in the pathogenesis of Streptococcus equi subspecies equi (S. equi), a host-restricted pathogen responsible for the highly contagious equine disease known as strangles. This disease elicits a significant inflammatory response, leading to symptoms such as lymphadenopathy of the head and neck, fever, nasal discharge, and decreased appetite, particularly in young horses whose immune systems are still developing. Understanding EAG protein structure is vital, as EAG facilitates the binding of various host proteins, which can influence the immune response and the overall pathogenicity of S. equi. Using anti-EAG antibody (C-9) in research can provide insights into the mechanisms of strangles and potentially develop strategies for prevention and treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EAG Antibody (C-9) References:
- Streptococcus equi with truncated M-proteins isolated from outwardly healthy horses. | Chanter, N., et al. 2000. Microbiology (Reading). 146 (Pt 6): 1361-1369. PMID: 10846214
- The molecular basis of Streptococcus equi infection and disease. | Harrington, DJ., et al. 2002. Microbes Infect. 4: 501-10. PMID: 11932201
- The pathogenic equine streptococci. | Timoney, JF. 2004. Vet Res. 35: 397-409. PMID: 15236673
- Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles. | Davidson, A., et al. 2008. J Vet Diagn Invest. 20: 457-62. PMID: 18599850
- Genomic evidence for the evolution of Streptococcus equi: host restriction, increased virulence, and genetic exchange with human pathogens. | Holden, MT., et al. 2009. PLoS Pathog. 5: e1000346. PMID: 19325880
- Streptococcus equi: a pathogen restricted to one host. | Waller, AS., et al. 2011. J Med Microbiol. 60: 1231-1240. PMID: 21757503
- Streptococcus equi subspecies equi infection (strangles) in horses. | Boyle, A. 2011. Compend Contin Educ Vet. 33: E1-7; quiz E8. PMID: 21870348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EAG Antibody (C-9) | sc-377236 | 200 µg/ml | $316.00 | |||
EAG Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-529993 | 200 µg Ab; 10 µg BP | $354.00 | |||
EAG Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-523245 | 200 µg Ab, 40 µg BP | $354.00 | |||
EAG Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-543808 | 200 µg Ab; 20 µg BP | $354.00 | |||
EAG Antibody (C-9) AC | sc-377236 AC | 500 µg/ml, 25% agarose | $416.00 | |||
EAG Antibody (C-9) HRP | sc-377236 HRP | 200 µg/ml | $316.00 | |||
EAG Antibody (C-9) FITC | sc-377236 FITC | 200 µg/ml | $330.00 | |||
EAG Antibody (C-9) PE | sc-377236 PE | 200 µg/ml | $343.00 | |||
EAG Antibody (C-9) Alexa Fluor® 488 | sc-377236 AF488 | 200 µg/ml | $357.00 | |||
EAG Antibody (C-9) Alexa Fluor® 546 | sc-377236 AF546 | 200 µg/ml | $357.00 | |||
EAG Antibody (C-9) Alexa Fluor® 594 | sc-377236 AF594 | 200 µg/ml | $357.00 | |||
EAG Antibody (C-9) Alexa Fluor® 647 | sc-377236 AF647 | 200 µg/ml | $357.00 | |||
EAG Antibody (C-9) Alexa Fluor® 680 | sc-377236 AF680 | 200 µg/ml | $357.00 | |||
EAG Antibody (C-9) Alexa Fluor® 790 | sc-377236 AF790 | 200 µg/ml | $357.00 |