E6-AP Antibody (F-1): sc-166688

E6-AP Antibody (F-1) is a mouse monoclonal IgG2a light chain antibody that detects E6-AP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E6-AP, also known as ubiquitin protein ligase E3A (UBE3A), plays a crucial role in the ubiquitin-mediated proteolytic pathway, which is essential for regulating protein degradation via the 26S proteasome. This process is vital for maintaining cellular homeostasis and controlling various cellular functions, including the cell cycle and apoptosis. E6-AP facilitates the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to target proteins, a mechanism that is particularly important for the degradation of key regulatory proteins such as the tumor suppressor p53. The interaction between E6-AP and p53 is significant because this interaction not only regulates p53 levels but also influences cellular responses to stress and DNA damage. Disruptions in E6-AP function, often due to genetic mutations, can lead to the accumulation of p53 in the cytoplasm, contributing to the pathogenesis of Angelman syndrome, a neurodevelopmental disorder characterized by severe motor dysfunction and cognitive impairment. Anti-E6-AP antibody (F-1) is therefore an invaluable tool for researchers studying the intricate mechanisms of protein regulation and the implications of E6-AP in various diseases.

The preferred E6-AP antibody is E6-AP Antibody (E-4): sc-166689.