E4BP4 Antibody (B-1): sc-137163

E4BP4 Antibody (B-1) is a mouse monoclonal IgG1 antibody that detects E4BP4 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E4BP4, also known as NFIL3, is a crucial transcriptional repressor belonging to the basic leucine zipper (bZIP) transcription factor family, which plays a significant role in regulating gene expression. E4BP4 is primarily located in the nucleus, where E4BP4 binds to specific DNA sequences, including the E4 ATF, to modulate the transcription of target genes. Nuclear localization allows E4BP4 to compete with other transcription factors, such as PAR proteins, for binding to DNA, thereby influencing circadian rhythms and cellular responses to various signals. E4BP4′s expression is phase-dependent, correlating with the circadian cycle, which underscores E4BP4′s importance in maintaining the rhythmic expression of genes involved in metabolic processes and cellular homeostasis. Additionally, E4BP4 is implicated in the interleukin-3-mediated signaling pathway, which is essential for the survival of B cell progenitors, highlighting E4BP4′s role in immune system regulation. The gene encoding human E4BP4 is located on chromosome 9q22, further emphasizing E4BP4′s significance in human biology and disease.

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E4BP4 Antibody (B-1) References:

1. Negative control of circadian clock regulator E4BP4 by casein kinase Iepsilon-mediated phosphorylation.  |  Doi, M., et al. 2004. Curr Biol. 14: 975-80. PMID: 15182670
2. E4BP4 overexpression: a protective mechanism in CD4+ T cells from SLE patients.  |  Zhao, M., et al. 2013. J Autoimmun. 41: 152-60. PMID: 23340290
3. The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression.  |  Male, V., et al. 2014. J Exp Med. 211: 635-42. PMID: 24663216
4. Correlation of glucocorticoid-mediated E4BP4 upregulation with altered expression of pro- and anti-apoptotic genes in CEM human lymphoblastic leukemia cells.  |  Beach, JA., et al. 2014. Biochem Biophys Res Commun. 451: 382-8. PMID: 25101525
5. E4BP4 is a repressor of epigenetically regulated SOSTDC1 expression in breast cancer cells.  |  Rawat, A., et al. 2014. Cell Oncol (Dordr). 37: 409-19. PMID: 25338303
6. E4BP4 is an insulin-induced stabilizer of nuclear SREBP-1c and promotes SREBP-1c-mediated lipogenesis.  |  Tong, X., et al. 2016. J Lipid Res. 57: 1219-30. PMID: 27252523
7. Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.  |  Kostrzewski, T., et al. 2018. J Immunol. 200: 1370-1381. PMID: 29311361
8. E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases.  |  Karthik, IP., et al. 2018. J Biol Chem. 293: 5624-5635. PMID: 29467226
9. E4BP4 promotes thyroid cancer proliferation by modulating iron homeostasis through repression of hepcidin.  |  Zhou, Q., et al. 2018. Cell Death Dis. 9: 987. PMID: 30250199
10. The transcription factor E4bp4 regulates the expression and activity of Cyp3a11 in mice.  |  Tong, Y., et al. 2019. Biochem Pharmacol. 163: 215-224. PMID: 30796914

The preferred E4BP4 antibody is E4BP4 Antibody (C-6): sc-374451.