E4BP4 Antibody (C-6): sc-374451

E4BP4 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects E4BP4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-E4BP4 antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E4BP4, also known as NFIL3, functions as a crucial transcriptional repressor belonging to the basic leucine zipper (bZIP) transcription factor family, playing a significant role in regulating gene expression. E4BP4 primarily localizes in the nucleus, binding to specific DNA sequences, including E4 ATF, and competes with other transcription factors for binding sites, thereby influencing transcription of circadian rhythm-related genes. E4BP4′s expression follows tight regulation by the circadian cycle, modulating genes activated by PAR transcription regulators, which maintains proper circadian rhythms and metabolic processes. E4BP4 participates in an interleukin-3-mediated signaling pathway supporting B cell progenitor survival, highlighting a crucial role in immune system function. Human E4BP4 gene resides on chromosome 9q22, demonstrating evolutionary conservation and relevance across species.

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E4BP4 Antibody (C-6) References:

1. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism.  |  Mitsui, S., et al. 2001. Genes Dev. 15: 995-1006. PMID: 11316793
2. E4BP4 expression is regulated by the t(17;19)-associated oncoprotein E2A-HLF in pro-B cells.  |  Yeung, J., et al. 2004. Br J Haematol. 125: 560-7. PMID: 15147370
3. Transcriptional repression by a novel member of the bZIP family of transcription factors.  |  Cowell, IG., et al. 1992. Mol Cell Biol. 12: 3070-7. PMID: 1620116
4. The basic leucine zipper transcription factor E4BP4 is essential for natural killer cell development.  |  Gascoyne, DM., et al. 2009. Nat Immunol. 10: 1118-24. PMID: 19749763
5. The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells.  |  Motomura, Y., et al. 2011. Nat Immunol. 12: 450-9. PMID: 21460847
6. E4BP4 facilitates glucocorticoid-evoked apoptosis of human leukemic CEM cells via upregulation of Bim.  |  Beach, JA., et al. 2011. J Mol Signal. 6: 13. PMID: 21975218
7. Recruitment of histone methyltransferase G9a mediates transcriptional repression of Fgf21 gene by E4BP4 protein.  |  Tong, X., et al. 2013. J Biol Chem. 288: 5417-25. PMID: 23283977
8. Glucocorticoid-mediated co-regulation of RCAN1-1, E4BP4 and BIM in human leukemia cells susceptible to apoptosis.  |  Saenz, GJ., et al. 2015. Biochem Biophys Res Commun. 463: 1291-6. PMID: 26102033
9. Transcriptional repression by the human bZIP factor E4BP4: definition of a minimal repression domain.  |  Cowell, IG. and Hurst, HC. 1994. Nucleic Acids Res. 22: 59-65. PMID: 8127655
10. Protein-protein interaction between the transcriptional repressor E4BP4 and the TBP-binding protein Dr1.  |  Cowell, IG. and Hurst, HC. 1996. Nucleic Acids Res. 24: 3607-13. PMID: 8836190
11. Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes.  |  Ikushima, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 2609-14. PMID: 9122243
12. A YAC-based transcript map of human chromosome 9q22.1-q22.3 encompassing the loci for hereditary sensory neuropathy type I and multiple self-healing squamous epithelioma.  |  Blair, IP., et al. 1998. Genomics. 51: 277-81. PMID: 9722951

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E4BP4 Antibody (C-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-E4BP4 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E4BP4 Antibody (C-6) Specifications

Alternate Product Name: E4BP4

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 425-462

Epitope Target: E4BP4

Epitope Species: human

Product Reactivity/Detection: E4BP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for E4BP4 Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent