E2F-5 Antibody (C-8): sc-374268

E2F-5 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects E2F-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-E2F-5 antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2F-5 plays a crucial role in regulating the cell cycle, particularly in the transition from G1 phase to S phase, by controlling the expression of genes necessary for DNA synthesis and cell proliferation. Such regulation maintains proper cell growth and division, while E2F-5 dysregulation can lead to uncontrolled cell proliferation, contributing to tumorigenesis. E2F-5 belongs to a larger family of E2F transcription factors, including E2F-1, E2F-2, E2F-3, E2F-4, E2F-6, and E2F-7, each forming heterodimers with DP-1 protein to create active transcriptional regulatory complexes. E2F-5′s interaction with other proteins, including retinoblastoma protein (Rb), underscores E2F-5′s importance in cell cycle regulation and highlights potential implications in cancer research and therapeutic development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

E2F-5 Antibody (C-8) References:

1. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
2. Interplay between the retinoblastoma related pRb2/p130 and E2F-4 and -5 in relation to JCV-TAg.  |  Caracciolo, V., et al. 2007. J Cell Physiol. 212: 96-104. PMID: 17385710
3. The E2F transcription factor is a cellular target for the RB protein.  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
4. The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein.  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
5. Molecular and functional characterisation of E2F-5, a new member of the E2F family.  |  Buck, V., et al. 1995. Oncogene. 11: 31-8. PMID: 7542760
6. E2F-5, a new E2F family member that interacts with p130 in vivo.  |  Hijmans, EM., et al. 1995. Mol Cell Biol. 15: 3082-9. PMID: 7760804
7. E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle.  |  Sardet, C., et al. 1995. Proc Natl Acad Sci U S A. 92: 2403-7. PMID: 7892279
8. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
9. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
10. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
11. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
12. E2F-6, a member of the E2F family that can behave as a transcriptional repressor.  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2F-5 Antibody (C-8) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-E2F-5 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2F-5 Antibody (C-8) Specifications

Alternate Product Name: E2F-5

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 303-331

Epitope Target: E2F-5

Epitope Species: human

Product Reactivity/Detection: E2F-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for E2F-5 Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred E2F-4 antibody is E2F-4 Antibody (RK-13): sc-511.