E2F-4 Antibody (WUF2): sc-69685
- E2F-4 Antibody (WUF2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against recombinant E2F-4 of human origin
- recommended for detection of E2F-4 of mouse, rat and human origin by WB and IP
- See E2F-4 (D-7): sc-398543 for E2F-4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for E2F-4 Antibody (WUF2) for WB applications. These reagents are now offered in bundles with E2F-4 Antibody (WUF2) (see ordering information below).
E2F-4 Antibody (WUF2) is a mouse monoclonal IgG antibody that detects E2F-4 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). E2F-4 is a crucial member of the E2F family of transcription factors, which are integral to the regulation of the cell cycle, particularly during the transition from the G1 phase to the S phase. E2F-4 functions primarily as a transcriptional repressor, playing a vital role in controlling the expression of genes necessary for cell proliferation and differentiation. E2F-4 forms heterodimers with the DP-1 protein, which is essential for the formation of an active transcriptional complex that regulates target gene expression. Dysregulation of E2F-4 can lead to uncontrolled cell growth and has been implicated in various cancers, making E2F-4 a significant focus of research in understanding tumorigenesis. Anti-E2F-4 antibody (WUF2) is an invaluable tool for studying the mechanisms of cell cycle regulation and potential therapeutic targets in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E2F-4 Antibody (WUF2) References:
- Distinct phosphorylation events regulate p130- and p107-mediated repression of E2F-4. | Farkas, T., et al. 2002. J Biol Chem. 277: 26741-52. PMID: 12006580
- Physical and functional interaction of HIV-1 Tat with E2F-4, a transcriptional regulator of mammalian cell cycle. | Ambrosino, C., et al. 2002. J Biol Chem. 277: 31448-58. PMID: 12055184
- E2F-4 mutation in hereditary non-polyposis colorectal cancer. | Moriyama, H., et al. 2002. J Exp Clin Cancer Res. 21: 185-9. PMID: 12148576
- Expression of E2F-4 gene in colorectal adenocarcinoma and corresponding covering mucosa: an immunohistochemistry, image analysis, and immunoblot study. | Mady, HH., et al. 2002. Appl Immunohistochem Mol Morphol. 10: 225-30. PMID: 12373148
- Inhibition of pituitary tumors in Rb mutant chimeras through E2f4 loss reveals a key suppressive role for the pRB/E2F pathway in urothelium and ganglionic carcinogenesis. | Parisi, T., et al. 2009. Oncogene. 28: 500-8. PMID: 18997819
- Functional impact of colorectal cancer-associated mutations in the transcription factor E2F4. | Paquin, MC., et al. 2013. Int J Oncol. 43: 2015-22. PMID: 24100580
- Knockdown of E2F4 suppresses the growth of ovarian cancer cells through the cell cycle pathway. | Kim, J., et al. 2021. Int J Clin Exp Pathol. 14: 866-874. PMID: 34527129
- E2F4-Based Gene Therapy Mitigates the Phenotype of the Alzheimer's Disease Mouse Model 5xFAD. | López-Sánchez, N., et al. 2021. Neurotherapeutics. 18: 2484-2503. PMID: 34766258
- A Positive Feedback Loop of E2F4-Mediated Activation of MNX1 Regulates Tumour Progression in Colorectal Cancer. | Li, JK., et al. 2023. J Cancer. 14: 2739-2750. PMID: 37779874
- Sequence of a human E2F-4 pseudogene with shortened AGC trinucleotide repeats. | Cheng, CM., et al. 1997. Kaohsiung J Med Sci. 13: 448-52. PMID: 9260465
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2F-4 Antibody (WUF2) | sc-69685 | 200 µg/ml | $322.00 | |||
E2F-4 Antibody (WUF2): m-IgG Fc BP-HRP Bundle | sc-539220 | 200 µg Ab; 10 µg BP | $361.00 | |||
E2F-4 Antibody (WUF2): m-IgG1 BP-HRP Bundle | sc-541334 | 200 µg Ab; 20 µg BP | $361.00 |