E2A CRISPR/Cas9 KO Plasmid (h): sc-423303. Western blot analysis of human E2A expression in empty vector control (A) and E2A CRISPR/Cas9 KO Plasmid trans-fected (B) NIH/3T3 whole cell lysates. Blot probed with E2A (Yae): sc-416. Actin (I-19): sc-1616 used as specificity and loading control.
E2A CRISPR Plasmids (m)
- Target species: mouse
- CRISPR/Cas9 KO Plasmids consists of E2A-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using E2A Antibody (Yae): sc-416
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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CRISPR/Cas9 KO Plasmid
Recommended Control Products
SEE ALSO...
E2A Antibodies for analysis of cellular responses to E2A CRISPR Products
Learn more about the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited/Label License. Click here for details
Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- E2A CRISPR/Cas9 Knockout (KO) Plasmid (m) consists of a pool of three plasmids each encoding the Cas9 nuclease and a E2A-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
- gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- **** NOTE: No corresponding HDR is available for this Knockout Plasmid. **** Please use sc-423303-KO-2 and sc-423303-HDR-2 if selection of cells using puromycin resistance is desired.
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: E2A Antibody (Yae): sc-416
See Protocol Online.
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2A CRISPR/Cas9 KO Plasmid (m) | sc-423303 | 20 µg | $397.00 |