Date published: 2025-10-15

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E-cadherin Antibody (5H9): sc-52327

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Datasheets
  • E-cadherin Antibody (5H9) is a mouse monoclonal IgG1, cited in 11 publications, provided at 50 µg/0.5 ml
  • raised against affinity purified 80 kDa extracellular fragments of E-cadherin of A-431 vulva carcinoma cells of human origin
  • recommended for detection of E-Cadherin of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See E-cadherin (G-10): sc-8426 for E-cadherin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for E-cadherin Antibody (5H9). This work is in progress.

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E-cadherin Antibody (5H9) is a mouse monoclonal IgG1 antibody that detects E-cadherin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. E-cadherin plays a crucial role in maintaining cell-cell adhesion, which is essential for the integrity of epithelial tissues and the regulation of morphogenetic processes. E-cadherin is primarily located at the cell membrane, where adherens junctions form to connect adjacent cells, thereby contributing to tissue architecture and stability. E-cadherin′s function is vital for preventing tumor metastasis, as loss of expression often associates with increased invasiveness of cancer cells. E-cadherin′s extracellular domain interacts with other cadherins and cytoplasmic proteins, such as β-catenin, which are involved in signaling pathways that regulate cell proliferation and differentiation. Anti-E-cadherin antibody (5H9) enables researchers to gain insights into cell adhesion mechanisms and E-cadherin′s implications in various diseases, particularly in cancer research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

E-cadherin Antibody (5H9)

sc-52327
50 µg/0.5 ml
$316.00

I would like to use E-cadherin (5H9): sc-52327 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-12
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Rated 1 out of 5 by from Doesn't seem to work as advertisedOur end user said that the antibody did not work as advertised and they did not have time to trouble shoot it.
Date published: 2018-08-13
Rated 4 out of 5 by from Good WB data with in LNCaP whole cell lysateGood WB data with in LNCaP whole cell lysate. -SCBT QC
Date published: 2015-02-13
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E-cadherin Antibody (5H9) is rated 2.5 out of 5 by 2.
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