dystrophin Antibody (7A10): sc-47760

Dystrophin Antibody (7A10) is a mouse monoclonal IgG1 kappa light chain antibody that detects dystrophin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-dystrophin antibody (7A10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dystrophin plays a crucial role in the dystrophin-glycoprotein complex (DGC), which connects the F-actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, thereby maintaining the structural integrity of muscle cells during contraction. A deficiency in dystrophin is a primary contributor to Duchenne (DMD) and Becker (BMD) muscular dystrophies, highlighting dystrophin′s importance in muscle function and health. The human dystrophin gene spans 2.4 megabases, contains over 80 exons, and produces a 14 kb mRNA that is regulated by at least eight independent tissue-specific promoters and two polyadenylation sites. Notably, dystrophin mRNA can undergo differential splicing, resulting in a variety of transcripts that encode a diverse set of proteins. Dystrophin constitutes approximately 0.002% of total striated muscle protein and is localized to triadic junctions in skeletal muscle, where dystrophin is believed to play a significant role in calcium ion homeostasis and force transmission, making dystrophin essential for proper muscle function.

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dystrophin Antibody (7A10) References:

1. Muscular dystrophies involving the dystrophin-glycoprotein complex: an overview of current mouse models.  |  Durbeej, M. and Campbell, KP. 2002. Curr Opin Genet Dev. 12: 349-61. PMID: 12076680
2. Dystrophin-glycoprotein complex: post-translational processing and dystroglycan function.  |  Michele, DE. and Campbell, KP. 2003. J Biol Chem. 278: 15457-60. PMID: 12556455
3. Skeletal muscle signaling pathway through the dystrophin glycoprotein complex and Rac1.  |  Oak, SA., et al. 2003. J Biol Chem. 278: 39287-95. PMID: 12885773
4. Convergent regulation of skeletal muscle Ca2+ channels by dystrophin, the actin cytoskeleton, and cAMP-dependent protein kinase.  |  Johnson, BD., et al. 2005. Proc Natl Acad Sci U S A. 102: 4191-6. PMID: 15753322
5. Molecular extensibility of mini-dystrophins and a dystrophin rod construct.  |  Bhasin, N., et al. 2005. J Mol Biol. 352: 795-806. PMID: 16139300
6. Dystrophin glycoprotein complex dysfunction: a regulatory link between muscular dystrophy and cancer cachexia.  |  Acharyya, S., et al. 2005. Cancer Cell. 8: 421-32. PMID: 16286249
7. Dystrophin is required for appropriate retrograde control of neurotransmitter release at the Drosophila neuromuscular junction.  |  van der Plas, MC., et al. 2006. J Neurosci. 26: 333-44. PMID: 16399704
8. Dystrophin and utrophin bind actin through distinct modes of contact.  |  Rybakova, IN., et al. 2006. J Biol Chem. 281: 9996-10001. PMID: 16478721
9. Dissecting the signaling and mechanical functions of the dystrophin-glycoprotein complex.  |  Judge, LM., et al. 2006. J Cell Sci. 119: 1537-46. PMID: 16569668
10. Dystrophin in the Neonatal and Adult Rat Intestine.  |  Lionarons, JM., et al. 2021. Life (Basel). 11: PMID: 34833031
11. Dystrophin, its gene, and the dystrophinopathies.  |  Roberts, RG. 1995. Adv Genet. 33: 177-231. PMID: 7484453
12. Muscular dystrophies and the dystrophin-glycoprotein complex.  |  Straub, V. and Campbell, KP. 1997. Curr Opin Neurol. 10: 168-75. PMID: 9146999

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dystrophin Antibody (7A10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-dystrophin Antibody (7A10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dystrophin Antibody (7A10) Specifications

Alternate Product Name: dystrophin

Product Clone Name: 7A10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3200-3684

Epitope Target: dystrophin

Epitope Species: human

Product Reactivity/Detection: an epitope corresponding to amino acids 3558-3684 of dystrophin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: fish

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for dystrophin Antibody (7A10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin