Dyskerin Antibody (H-3) recommended for detection of Dyskerin of mouse, rat and human origin by WB, IP, IF and ELISA

Dyskerin Antibody (H-3): sc-373956

Dyskerin Antibody (H-3) is rated 4.5 out of 5 by 8.
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Datasheets

    • Anti-Dyskerin Antibody (H-3) is a mouse monoclonal IgG2a κ Dyskerin antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 171-470 mapping within an internal region of Dyskerin of human origin
    • Anti-Dyskerin Antibody (H-3) is recommended for detection of Dyskerin of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Dyskerin Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-373956 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dyskerin (H-3): sc-373956.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Dyskerin Antibody (H-3) for WB applications. This reagent is now offered in a bundle with Dyskerin Antibody (H-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

We would like to test your Dyskerin Antibody (H-3): sc-373956 in our research in Arabidopsis plants, could you please send me a free sample

Asked by: jsaez
Thank you for the question. Our Technical Service Dept. will contact you shortly.
Answered by: Technical Support Europe
Date published: 2018-01-19

What is the recommended fixation method for immunofluorescence staining with Dyskerin (H-3): sc-373956 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from ChIP at telomeres This antibody worked well for telomeric ChIP in 293T cells.
Date published: 2018-02-26
Rated 5 out of 5 by from Works for WB/IF/PLA Bought this antibody (DKC1) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:1500 and IF/PLA 1:250. Also used alongside irradiated samples and worked like a charm.
Date published: 2017-08-15
Rated 5 out of 5 by from nice antibody can recognize dyskerin in different lung cancer cells
Date published: 2017-04-04
Rated 5 out of 5 by from Excellent choice for Dyskerin! This antibody gives beautiful bands on Western blots, especially when used with the Mouse IgG binding protein reagent! Strong and reliable, it's a win-win!
Date published: 2017-01-18
Rated 4 out of 5 by from Great for IF Works well for indirect immunofluorescence on PFA fixed HeLa cells, but was unsuccessful on westerns of whole cell extracts of the same cells.
Date published: 2016-12-27
Rated 4 out of 5 by from Robust band seen in WB using HeLa nuclear extract Robust band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Beautiful immunofluorescence nucleolar staining Beautiful immunofluorescence nucleolar staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Strong band detected in WB using Raji whole Strong band detected in WB using Raji whole cell lysate. -SCBT QC
Date published: 2015-04-11
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