Dysadherin Antibody (E-12): sc-515254
- Dysadherin Antibody (E-12) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 30-51 within an N-terminal extracellular domain of Dysadherin of human origin
- Dysadherin Antibody (E-12) is recommended for detection of Dysadherin isoform 1 of human origin by WB, IP, IF and ELISA
- Anti-Dysadherin Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dysadherin Antibody (E-12) for WB applications. These reagents are now offered in bundles with Dysadherin Antibody (E-12) (see ordering information below).
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SEE ALSO...
Dysadherin Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dysadherin of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dysadherin antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dysadherin, also known as FXYD domain-containing ion transport regulator 5, is a critical cancer-associated cell membrane glycoprotein that plays a significant role in regulating cell adhesion. Dysadherin functions by down-regulating E-cadherin expression, the primary mediator of cell-cell adhesion in epithelial tissues, through a posttranscriptional mechanism. This reduction in intercellular adhesion facilitates cancer cell metastasis, allowing invasion into surrounding tissues and spread to distant sites. Dysadherin is predominantly located in the cell membrane and is expressed in various human tissues, including the spleen, lung, skeletal muscle, and testis, mapping to human chromosome 19q13.2. Dysadherin′s ability to modulate cell adhesion highlights its importance in cancer biology and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dysadherin Antibody (E-12) References:
- Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis. | Ino, Y., et al. 2002. Proc Natl Acad Sci U S A. 99: 365-70. PMID: 11756660
- Prognostic significance of dysadherin expression in patients with non-small cell lung cancer. | Tamura, M., et al. 2005. J Thorac Cardiovasc Surg. 130: 740-5. PMID: 16153922
- Chemokine (C-C motif) ligand 2 mediates the prometastatic effect of dysadherin in human breast cancer cells. | Nam, JS., et al. 2006. Cancer Res. 66: 7176-84. PMID: 16849564
- Differential expression of dysadherin in papillary thyroid carcinoma and microcarcinoma: correlation with E-cadherin. | Batistatou, A., et al. 2008. Endocr Pathol. 19: 197-202. PMID: 18677652
- Prognostic significance of dysadherin and E-cadherin expression in patients with head and neck cancer treated by radiation therapy. | Muramatsu, H., et al. 2008. Anticancer Res. 28: 3859-64. PMID: 19192641
- Dysadherin expression in gastrointestinal stromal tumors (GISTs). | Liang, JF., et al. 2009. Pathol Res Pract. 205: 445-50. PMID: 19217217
- Expression of dysadherin and cytokeratin as prognostic indicators of disease-free survival in patients with stage I NSCLC. | Ono, K., et al. 2010. Anticancer Res. 30: 3273-8. PMID: 20944097
- Osteoblast-secreted factors enhance the expression of dysadherin and CCL2-dependent migration of renal carcinoma cells. | Schüler, Y., et al. 2012. Int J Cancer. 130: 288-99. PMID: 21328336
- Modulation of cell polarization by the Na+-K+-ATPase-associated protein FXYD5 (dysadherin). | Lubarski, I., et al. 2014. Am J Physiol Cell Physiol. 306: C1080-8. PMID: 24717576
- FXYD5 (Dysadherin) upregulation predicts shorter survival and reveals platinum resistance in high-grade serous ovarian cancer patients. | Tassi, RA., et al. 2019. Br J Cancer. 121: 584-592. PMID: 31434988
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dysadherin Antibody (E-12) | sc-515254 | 200 µg/ml | $322.00 | |||
Dysadherin Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-531324 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dysadherin Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-524987 | 200 µg Ab, 40 µg BP | $361.00 | |||
Dysadherin Antibody (E-12) AC | sc-515254 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Dysadherin Antibody (E-12) HRP | sc-515254 HRP | 200 µg/ml | $322.00 | |||
Dysadherin Antibody (E-12) FITC | sc-515254 FITC | 200 µg/ml | $336.00 | |||
Dysadherin Antibody (E-12) PE | sc-515254 PE | 200 µg/ml | $349.00 | |||
Dysadherin Antibody (E-12) Alexa Fluor® 488 | sc-515254 AF488 | 200 µg/ml | $364.00 | |||
Dysadherin Antibody (E-12) Alexa Fluor® 546 | sc-515254 AF546 | 200 µg/ml | $364.00 | |||
Dysadherin Antibody (E-12) Alexa Fluor® 594 | sc-515254 AF594 | 200 µg/ml | $364.00 | |||
Dysadherin Antibody (E-12) Alexa Fluor® 647 | sc-515254 AF647 | 200 µg/ml | $364.00 | |||
Dysadherin Antibody (E-12) Alexa Fluor® 680 | sc-515254 AF680 | 200 µg/ml | $364.00 | |||
Dysadherin Antibody (E-12) Alexa Fluor® 790 | sc-515254 AF790 | 200 µg/ml | $364.00 | |||
Dysadherin (E-12) Neutralizing Peptide | sc-515254 P | 100 µg/0.5 ml | $69.00 |