Dynamin I Antibody (D5): sc-12724

Dynamin I Antibody (D5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dynamin I protein of mouse, rat, human, canine, and avian origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Dynamin I antibody (D5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dynamin I plays a crucial role in endocytosis, synaptic transmission, and neurogenesis, making Dynamin I essential for cellular communication and maintenance of neuronal health. Dynamin I is primarily localized in the central nervous system, where Dynamin I is involved in the formation of clathrin-coated vesicles, a process vital for internalization of neurotransmitters and other signaling molecules. The structure of Dynamin I includes SH3 and proline-rich domains that facilitate Dynamin I interaction with various effector proteins, which are necessary for GTPase activity and subsequent endocytic function. Notably, Dynamin I is recruited to clathrin-coated pits through Dynamin I interaction with amphiphysin, a protein highly expressed in the brain, underscoring the importance of these interactions in regulation of synaptic vesicle recycling and neuronal signaling.

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Dynamin I Antibody (D5) References:

1. Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles.  |  Tomizawa, K., et al. 2003. J Cell Biol. 163: 813-24. PMID: 14623869
2. Dynamin I phosphorylation and the control of synaptic vesicle endocytosis.  |  Smillie, KJ. and Cousin, MA. 2005. Biochem Soc Symp. 87-97. PMID: 15649133
3. Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis.  |  Anggono, V., et al. 2006. Nat Neurosci. 9: 752-60. PMID: 16648848
4. Interaction of dynamin I with NAP-22, a neuronal protein enriched in the presynaptic region.  |  Ueno, S., et al. 2018. Neurosci Lett. 675: 59-63. PMID: 29604406
5. Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences.  |  Scaife, R., et al. 1994. EMBO J. 13: 2574-82. PMID: 8013457
6. Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues.  |  Cook, TA., et al. 1994. Proc Natl Acad Sci U S A. 91: 644-8. PMID: 8290576
7. Differential expression and regulation of multiple dynamins.  |  Sontag, JM., et al. 1994. J Biol Chem. 269: 4547-54. PMID: 8308025
8. A binding site for SH3 domains targets dynamin to coated pits.  |  Shpetner, HS., et al. 1996. J Biol Chem. 271: 13-6. PMID: 8550547
9. Role of the basic, proline-rich region of dynamin in Src homology 3 domain binding and endocytosis.  |  Okamoto, PM., et al. 1997. J Biol Chem. 272: 11629-35. PMID: 9111080
10. The SH3 domain of amphiphysin binds the proline-rich domain of dynamin at a single site that defines a new SH3 binding consensus sequence.  |  Grabs, D., et al. 1997. J Biol Chem. 272: 13419-25. PMID: 9148966
11. Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain.  |  Wigge, P., et al. 1997. Curr Biol. 7: 554-60. PMID: 9259551
12. The role of the PH domain and SH3 binding domains in dynamin function.  |  Scaife, RM. and Margolis, RL. 1997. Cell Signal. 9: 395-401. PMID: 9376220

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dynamin I Antibody (D5) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Dynamin I Antibody (D5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dynamin I Antibody (D5) Specifications

Alternate Product Name: Dynamin I

Product Clone Name: D5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-750

Epitope Target: Dynamin I

Epitope Species: human

Product Reactivity/Detection: Dynamin I

Species Reactivity/Detection: mouse, rat, human, canine, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Dynamin I Antibody (D5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin