Dynamin I Antibody (4E67): sc-58260

Dynamin I Antibody (4E67) is a mouse monoclonal IgG1 antibody that detects Dynamin I in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Dynamin I plays a crucial role in endocytosis, which is essential for cellular communication and nutrient uptake, particularly in the central nervous system where Dynamin I is predominantly localized. Dynamin I is vital for the formation of clathrin-coated vesicles, a key step in the internalization of membrane proteins and receptors. The structure of Dynamin I includes a GTPase domain responsible for enzymatic activity, as well as SH3 and proline-rich domains that facilitate interactions with various effector proteins, such as amphiphysin, which is highly expressed in the brain. These interactions are critical for the recruitment of Dynamin I to clathrin-coated pits, enabling efficient vesicle budding from the plasma membrane. Dynamin I′s regulation of endocytosis not only impacts synaptic transmission but also plays a significant role in neurogenesis, highlighting Dynamin I′s importance in maintaining neuronal health and function. Anti-Dynamin I antibody (4E67) is an invaluable tool for researchers studying these processes and the underlying mechanisms of neurobiology.

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Dynamin I Antibody (4E67) References:

1. Activation of dynamin I gene expression by Sp1 and Sp3 is required for neuronal differentiation of N1E-115 cells.  |  Yoo, J., et al. 2002. J Biol Chem. 277: 11904-9. PMID: 11809758
2. Syndapin I and endophilin I bind overlapping proline-rich regions of dynamin I: role in synaptic vesicle endocytosis.  |  Anggono, V. and Robinson, PJ. 2007. J Neurochem. 102: 931-43. PMID: 17437541
3. Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles.  |  Clayton, EL., et al. 2010. Nat Neurosci. 13: 845-51. PMID: 20526333
4. SH3 Domains Differentially Stimulate Distinct Dynamin I Assembly Modes and G Domain Activity.  |  Krishnan, S., et al. 2015. PLoS One. 10: e0144609. PMID: 26659814
5. Pyrimidine-Based Inhibitors of Dynamin I GTPase Activity: Competitive Inhibition at the Pleckstrin Homology Domain.  |  Odell, LR., et al. 2017. J Med Chem. 60: 349-361. PMID: 27997171
6. Phosphorylation of dynamin I and synaptic-vesicle recycling.  |  Robinson, PJ., et al. 1994. Trends Neurosci. 17: 348-53. PMID: 7526507
7. Calcineurin inhibition of dynamin I GTPase activity coupled to nerve terminal depolarization.  |  Liu, JP., et al. 1994. Science. 265: 970-3. PMID: 8052858
8. Dynamin I is a Ca(2+)-sensitive phospholipid-binding protein with very high affinity for protein kinase C.  |  Liu, JP., et al. 1994. J Biol Chem. 269: 21043-50. PMID: 8063723
9. Calcium binds dynamin I and inhibits its GTPase activity.  |  Liu, JP., et al. 1996. J Neurochem. 66: 2074-81. PMID: 8780038
10. Ubiquitously expressed dynamin-II has a higher intrinsic GTPase activity and a greater propensity for self-assembly than neuronal dynamin-I.  |  Warnock, DE., et al. 1997. Mol Biol Cell. 8: 2553-62. PMID: 9398675

The preferred Dynamin I antibody is Dynamin I Antibody (D5): sc-12724.