Dvl-3 Antibody (G-7) recommended for detection of Dvl-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Dvl-3 Antibody (G-7): sc-271295

Dvl-3 Antibody (G-7) is rated 4.8 out of 5 by 6.
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Datasheets

    • Anti-Dvl-3 Antibody (G-7) is a mouse monoclonal IgG1 (kappa light chain) Dvl-3 antibody provided at 200 µg/ml
    • raised against amino acids 606-665 mapping near the C-terminus of Dishevelled-3 of human origin
    • Anti-Dvl-3 Antibody (G-7) is recommended for detection of Dvl-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Dvl-3 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dvl-3 (G-7): sc-271295.

Can Dvl-3 (G-7): sc-271295 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, Dvl-3 (G-7): sc-271295 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Great! Applied for free 10 µg sample two weeks ago, Direct western blot(starting dilution 1:100)analysis of Dvl-3 expression in mouse.
Date published: 2017-09-26
Rated 5 out of 5 by from This antibody worked well! IHC of paraffin-embedded human tissue showed nice staining with no background.I diluted the antibody one hundred times in my experiment.I am satisfied with this antibody!
Date published: 2017-05-05
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human uterine and cervix tissue. -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Robust band in western blot with transfected Robust band in western blot with transfected lysates. -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Band at expected molecular weight in western Band at expected molecular weight in western blot analysis of Dvl-3 expression in ZR-75-1 whole cell lysate. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from WB: strong WB: strong, clean band in human heart tissue extract. -SCBT QC
Date published: 2015-01-07
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