Dvl-3 Antibody (4D3) recommended for detection of Dvl-3 of mouse, rat and human origin by WB and IP

Dvl-3 Antibody (4D3): sc-8027

Dvl-3 Antibody (4D3) is rated 4.9 out of 5 by 9.
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Datasheets

    • Dvl-3 Antibody (4D3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 607-704 mapping at the C-terminus of Dvl-3 of mouse origin
    • Anti-Dvl-3 Antibody (4D3) is recommended for detection of Dvl-3 of mouse, rat and human origin by WB and IP
    • Anti-Dvl-3 Antibody (4D3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dvl-3 (4D3): sc-8027.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from Dvl-3 (4D3), sc-8027 This antibody has worked perfectly for us in Western Blot on HUVEC lysates. It did not require any special optimization, but worked fine in 5% BSA at a suggested dilution (1:500). Upon stimulation with WNT3A ligand we detected an expected shift in bands.
Date published: 2018-12-13
Rated 5 out of 5 by from Excellent antibody Excellent for WB (1:2500), IP (2ug) and IF (1:300). Specific for Dvl3 isoform in endothelial cells
Date published: 2018-03-09
Rated 5 out of 5 by from Superb western Great for western blot applications;strong, specific signal
Date published: 2017-01-27
Rated 5 out of 5 by from Reliable Antibody, Great for WB Strong, clear signal with little to no background. Great for WB. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-13
Rated 5 out of 5 by from CoIP using HEK293 cells CoIP using HEK293 cells. -SCBT Publication Review
Date published: 2015-04-30
Rated 5 out of 5 by from Clean staining in paraffin embedded lung Clean staining in paraffin embedded lung adenocarcinoma and squamous cell carcinoma sections using 1:50 dilution. -SCBT Publication Review
Date published: 2015-04-16
Rated 4 out of 5 by from Successful immunoprecipitation using 4D3 Successful immunoprecipitation using 4D3 with Hek293T, Cos-7, and Cos-1 cells; verification via western blotting. -SCBT Publication Review
Date published: 2015-04-05
Rated 5 out of 5 by from Published western blot data in TC Published western blot data in TC-32, 5838, and SKES whole cell lysates. -SCBT Publication Review
Date published: 2015-02-08
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