Dvl-2 Antibody (10B5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 594-736 mapping at the C-terminus of Dvl-2 of mouse origin
Anti-Dvl-2 Antibody (10B5) is recommended for detection of Dvl-2 of mouse, rat and human origin by WB, IP, IF and ELISA
Dvl-2 Antibody (10B5) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for WB (RGB), IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dvl-2 (10B5): sc-8026.
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Mammalian homologs of the Drosophila dishevelled (Dsh) gene have been identified, including Dvl-1, Dvl-2 and Dvl-3. The mammalian dishevelled proteins contain three homologous domains, two of which are unrelated to any other known protein. The third region is homologous to the discs-large homology domain of Drosophila discs-large-1, a tumor suppressor protein. Like their Drosophila counterpart, the dishevelled proteins are thought to be involved in embryogenesis. Overexpression of Dvl-1 has been shown to inhibit the phosphorylation of tau by GSK-3β. This finding may prove to be important in Alzheimer's studies, which have shown that tau is hyperphosphorylated. In Drosophila, Dsh is a component of the frizzled signaling pathway. Both mammalian dishevelled and frizzled proteins are components of the Wnt signalling pathway.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What acrylamide gel percentage should I use to see the phosphorylated and non phosphorylated DVL2 forms (on -/+ WNT stimulated), on HEK293 and MEF lysates?
As anyone done this with the MOPS running buffer?
Asked by: Researcher37
Thank you for your question. You can find our recommended protocol for western blotting by following this link:
We recommend using either Running Buffer, 10X, 1 L sc-24949 or MOPS buffer sc-216096. For assistance with SDS PAGE please contact our technical service directly with the details of your setup.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
Is this Dvl-2 Antibody (10B5): sc-8026 available directly conjugated to HRP or fluorescent dyes?
Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected]
Alternatively, consider using our Dvl-2 (D-6): sc-390303, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/dvl-2-antibody-d-6)
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn