Dvl-2 Antibody (10B5) recommended for detection of Dvl-2 of mouse, rat and human origin by WB, IP, IF and ELISA

Dvl-2 Antibody (10B5): sc-8026

Dvl-2 Antibody (10B5) is rated 5.0 out of 5 by 9.
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Datasheets

    • Dvl-2 Antibody (10B5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 594-736 mapping at the C-terminus of Dvl-2 of mouse origin
    • Anti-Dvl-2 Antibody (10B5) is recommended for detection of Dvl-2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Dvl-2 Antibody (10B5) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for WB (RGB), IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dvl-2 (10B5): sc-8026.

What acrylamide gel percentage should I use to see the phosphorylated and non phosphorylated DVL2 forms (on -/+ WNT stimulated), on HEK293 and MEF lysates? As anyone done this with the MOPS running buffer? Thanks

Asked by: Researcher37
Thank you for your question. You can find our recommended protocol for western blotting by following this link: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting We recommend using either Running Buffer, 10X, 1 L sc-24949 or MOPS buffer sc-216096. For assistance with SDS PAGE please contact our technical service directly with the details of your setup.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Is this Dvl-2 Antibody (10B5): sc-8026 available directly conjugated to HRP or fluorescent dyes?

Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using our Dvl-2 (D-6): sc-390303, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/dvl-2-antibody-d-6)
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from Works great in WB Clean band in western blotting on 293t17 whole lysate. 1:1000, o/n 4°C in TBS+0.1% Tween20+5%NonFatDryMilk
Date published: 2019-11-22
Rated 5 out of 5 by from Good Dvl-2 detection The antibody is specific--confirmed with siRNA knockdown data--for mouse, rat, or human detection. Great choice for Dvl-2.
Date published: 2017-01-18
Rated 5 out of 5 by from Superb antibody Very nice western blot and IF results, highly specific
Date published: 2017-01-09
Rated 5 out of 5 by from Western blotting using multiple myeloma and B Western blotting using multiple myeloma and B-cell lines. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Successful knockdown detected using sc Successful knockdown detected using sc-8026, beautiful WB data using this mono. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic staining in methanol-fixed BT-20 cells. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Strong clean band in BT Strong clean band in BT-20 whole cell lysate by western blot. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Worked well for IP with HEK293T cells using Worked well for IP with HEK293T cells using 10B5 and protein A/G plus agarose. -SCBT Publication Review
Date published: 2015-01-28
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