Dvl-1/Dishevelled 1/DVL1 Antibody (3F12): sc-8025

Dvl-1 Antibody (3F12) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect dishevelled segment polarity protein 1 (Dvl-1) across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Dvl-1 is a pivotal component of the Wnt signaling pathway, which is essential for regulating embryonic development, cell proliferation, and differentiation. Located predominantly in the cytoplasm and associated with the plasma membrane, Dvl-1 acts as a crucial intermediary that transmits signals from Wnt receptors to downstream effectors, thereby orchestrating various cellular processes. Structurally, Dvl-1 comprises three conserved domains, the DIX, PDZ, and DEP domains, that facilitate interactions with key proteins such as Frizzled receptors and Axin, enabling the modulation of both canonical and non-canonical Wnt signaling pathways. Post-translational modifications, particularly phosphorylation, play a significant role in regulating Dvl-1′s activity and stability, making these modifications vital for accurate signal transduction and cellular function. Dvl-1 interacts with several proteins, including members of the Frizzled family and components of the β-catenin destruction complex, underscoring Dvl-1′s importance in maintaining cellular homeostasis and involvement in pathological conditions like cancer and neurodegenerative diseases. Anti-Dvl-1 antibody (3F12) is raised against amino acids 651-695 at the C-terminus of the mouse Dvl-1 protein. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, Dvl-1 (3F12) antibody provides versatile applications for researchers investigating the intricate mechanisms of the Wnt signaling pathway and its implications in developmental biology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) References:

1. Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.  |  Sussman, DJ., et al. 1994. Dev Biol. 166: 73-86. PMID: 7958461
2. Dishevelled is a component of the frizzled signaling pathway in Drosophila.  |  Krasnow, RE., et al. 1995. Development. 121: 4095-102. PMID: 8575310
3. Human homologue sequences to the Drosophila dishevelled segment-polarity gene are deleted in the DiGeorge syndrome.  |  Pizzuti, A., et al. 1996. Am J Hum Genet. 58: 722-9. PMID: 8644734
4. cDNA characterization and chromosomal mapping of two human homologues of the Drosophila dishevelled polarity gene.  |  Pizzuti, A., et al. 1996. Hum Mol Genet. 5: 953-8. PMID: 8817329
5. Isolation and characterization of mouse dishevelled-3.  |  Tsang, M., et al. 1996. Dev Dyn. 207: 253-62. PMID: 8922524
6. A frizzled homolog functions in a vertebrate Wnt signaling pathway.  |  Yang-Snyder, J., et al. 1996. Curr Biol. 6: 1302-6. PMID: 8939578
7. Human dishevelled genes constitute a DHR-containing multigene family.  |  Semënov, MV. and Snyder, M. 1997. Genomics. 42: 302-10. PMID: 9192851
8. Overexpression of the mouse dishevelled-1 protein inhibits GSK-3beta-mediated phosphorylation of tau in transfected mammalian cells.  |  Wagner, U., et al. 1997. FEBS Lett. 411: 369-72. PMID: 9271238

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) has 81 citations in a variety of scientific publications.

Primary antibodies like Anti-Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) Specifications

Alternate Product Name: DVL1

Product Clone Name: 3F12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 651-695

Epitope Target: Dvl-1

Epitope Species: mouse

Product Reactivity/Detection: Dvl-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 81 citations in various scientific publications

Available Conjugates/Forms for Dvl-1/Dishevelled 1/DVL1 Antibody (3F12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Dvl/Dishevelled antibody is Dvl/Dishevelled Antibody (B-4): sc-166303.