Dvl-1 Antibody (3F12) recommended for detection of Dvl-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Dvl-1 Antibody (3F12): sc-8025

Dvl-1 Antibody (3F12) is rated 5.0 out of 5 by 14.
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Datasheets

    • Anti-Dvl-1 Antibody (3F12) is a mouse monoclonal IgG2a (kappa light chain) Dvl-1 antibody provided at 200 µg/ml
    • raised against amino acids 651-695 mapping at the C-terminus of Dvl-1 of mouse origin
    • Anti-Dvl-1 Antibody (3F12) is recommended for detection of Dvl-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Dvl-1 Antibody (3F12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dvl-1 (3F12): sc-8025.

没有出现85的条带。。。。只出现35左右的条带。。。你们有遇见这种情况吗

Asked by: 哈哈许松
感谢您的提问。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-09-11

各位,你们用这个抗体,有出现30多的条带出现吗?

Asked by: 哈哈许松
感谢您的提问。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-09-11

I am using Dvl-1 Antibody (3F12): sc-8025 to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 5 out of 5 by from work great The antibody was perfect. No background detected, very strong signal.
Date published: 2017-09-22
Rated 5 out of 5 by from strong signal The Dvl-1 protein is highly expressed in the intestinal tract of Hirschsprung's disease tissue, and a strong signal is still obtained after dilution of 1:1000, this sample that can be used as a positive control,the specific band is at 85kda.
Date published: 2017-09-09
Rated 5 out of 5 by from Works great Excellent antibody! One single (and strong) band at the right molecular weight
Date published: 2017-07-21
Rated 5 out of 5 by from Western blot is satisfied No background detected. there were target bands detected by sc-8025,very strong signal.
Date published: 2017-07-08
Rated 5 out of 5 by from 强大 条带清楚,特异性强,单抗的效果还是不错的,荧光强度,稳定性都不错,我一直都用这个货号的抗体,继续关注
Date published: 2017-06-06
Rated 5 out of 5 by from good production The product quality is very good, and result of the band is very clear and strong even at a 1:800 dilution in western blot
Date published: 2017-05-01
Rated 5 out of 5 by from I am satisfied with this antibody Not long ago ,I used this antibody for IHC(P).In my experiment,I diluted the antibody one hundred times.And I got wonderful results.
Date published: 2017-03-29
Rated 5 out of 5 by from Excellent IHC staining Great ihc staining;strong signal; also good for wb
Date published: 2017-01-04
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