DP-1 Antibody (TFD-10): sc-53642

DP-1 Antibody (TFD-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects DP-1 in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-DP-1 antibody (TFD-10) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DP-1 plays a crucial role in forming E2F transcriptional regulatory complex, essential for cell cycle progression regulation, particularly during G1 and S phases. This complex forms when DP-1 heterodimerizes with E2F-1, enabling transcriptional activation of genes vital for cell proliferation. Dysregulation of E2F-DP-1 complex can lead to uncontrolled cell growth and is often implicated in various cancers. Understanding DP-1 function and interactions remains critical for developing targeted therapies aimed at restoring normal cell cycle regulation in tumorigenesis.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DP-1 Antibody (TFD-10) References:

1. E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.  |  Nevins, JR. 1992. Science. 258: 424-9. PMID: 1411535
2. Identification and characterization of novel isoforms of human DP-1: DP-1{alpha} regulates the transcriptional activity of E2F1 as well as cell cycle progression in a dominant-negative manner.  |  Ishida, H., et al. 2005. J Biol Chem. 280: 24642-8. PMID: 15863509
3. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
4. Pleiotropic effect of somatic mutations in the E2F subunit DP-1 gene in human cancer.  |  Munro, S., et al. 2014. Oncogene. 33: 3594-603. PMID: 23934193
5. Comprehensive Analysis Reveals the Potential Roles of Transcription Factor Dp-1 in Lung Adenocarcinoma.  |  Song, Y. and Ma, R. 2023. World J Oncol. 14: 205-223. PMID: 37350808
6. Cloning and characterization of human DP2, a novel dimerization partner of E2F.  |  Zhang, Y. and Chellappan, SP. 1995. Oncogene. 10: 2085-93. PMID: 7784053
7. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
8. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
9. Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation.  |  Xu, M., et al. 1994. Mol Cell Biol. 14: 8420-31. PMID: 7969176
10. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
11. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
12. The molecular basis of E2F-1/DP-1-induced S-phase entry and apoptosis.  |  Shan, B., et al. 1996. Cell Growth Differ. 7: 689-97. PMID: 8780882

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DP-1 Antibody (TFD-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-DP-1 Antibody (TFD-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DP-1 Antibody (TFD-10) Specifications

Alternate Product Name: DP-1

Product Clone Name: TFD-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 83-204

Epitope Target: DP-1

Epitope Species: human

Product Reactivity/Detection: DP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for DP-1 Antibody (TFD-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin