DOT1L1 Antibody (E-5): sc-374317

DOT1L1 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DOT1L1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DOT1L1 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DOT1L1, also known as DOT1L (DOT1-like, histone H3 methyltransferase), DOT1, or KMT4, consists of 1739 amino acids and is a homolog of the yeast DOT1 protein, which is crucial for maintaining telomeric silencing. DOT1L1 is primarily localized in the nucleus and is highly expressed in tissues such as the testis, lung, and kidney. As a histone methyltransferase, DOT1L1 plays a vital role in epigenetic regulation by transferring methyl groups from S-adenosyl-L-methionine to lysine residues on histones and other substrates, thereby influencing gene expression. DOT1L1 lacks the SET domain that is characteristic of most histone methyltransferases, suggesting a unique mechanism for enzymatic activity. DOT1L1 has been implicated in oncogenesis through interactions with several MLL-fusion partners associated with acute leukemia, highlighting significance in cancer biology. Alternative splicing of the DOT1L1 gene results in the expression of two isoforms, further diversifying functional roles in cellular processes.

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DOT1L1 Antibody (E-5) References:

1. Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain.  |  Feng, Q., et al. 2002. Curr Biol. 12: 1052-8. PMID: 12123582
2. Structure of the catalytic domain of human DOT1L, a non-SET domain nucleosomal histone methyltransferase.  |  Min, J., et al. 2003. Cell. 112: 711-23. PMID: 12628190
3. hDOT1L links histone methylation to leukemogenesis.  |  Okada, Y., et al. 2005. Cell. 121: 167-78. PMID: 15851025
4. Aldosterone-sensitive repression of ENaCalpha transcription by a histone H3 lysine-79 methyltransferase.  |  Zhang, W., et al. 2006. Am J Physiol Cell Physiol. 290: C936-46. PMID: 16236820
5. Dot1a-AF9 complex mediates histone H3 Lys-79 hypermethylation and repression of ENaCalpha in an aldosterone-sensitive manner.  |  Zhang, W., et al. 2006. J Biol Chem. 281: 18059-68. PMID: 16636056
6. Leukaemic transformation by CALM-AF10 involves upregulation of Hoxa5 by hDOT1L.  |  Okada, Y., et al. 2006. Nat Cell Biol. 8: 1017-24. PMID: 16921363
7. DOT1L/KMT4 recruitment and H3K79 methylation are ubiquitously coupled with gene transcription in mammalian cells.  |  Steger, DJ., et al. 2008. Mol Cell Biol. 28: 2825-39. PMID: 18285465
8. The recurrent SET-NUP214 fusion as a new HOXA activation mechanism in pediatric T-cell acute lymphoblastic leukemia.  |  Van Vlierberghe, P., et al. 2008. Blood. 111: 4668-80. PMID: 18299449
9. The diverse functions of Dot1 and H3K79 methylation.  |  Nguyen, AT. and Zhang, Y. 2011. Genes Dev. 25: 1345-58. PMID: 21724828
10. Integrative analysis reveals functional and regulatory roles of H3K79me2 in mediating alternative splicing.  |  Li, T., et al. 2018. Genome Med. 10: 30. PMID: 29665865
11. Computational modeling reveals key molecular properties and dynamic behavior of disruptor of telomeric silencing 1-like (DOT1L) and partnering complexes involved in leukemogenesis.  |  Stodola, TJ., et al. 2022. Proteins. 90: 282-298. PMID: 34414607
12. HOXA9 Regulome and Pharmacological Interventions in Leukemia.  |  Aryal, S. and Lu, R. 2024. Adv Exp Med Biol. 1459: 405-430. PMID: 39017854

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DOT1L1 Antibody (E-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-DOT1L1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DOT1L1 Antibody (E-5) Specifications

Alternate Product Name: DOT1L1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: DOT1L1

Epitope Species: human

Product Reactivity/Detection: DOT1L1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for DOT1L1 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent