Dok-7 Antibody (A-7): sc-390856

Dok-7 Antibody (A-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Dok-7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dok-7 (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dok-7 protein plays a pivotal role in neuromuscular synaptogenesis by interacting with muscle-specific receptor kinase (MuSK), which is essential for the formation of neuromuscular junctions and MuSK activation. This interaction facilitates acetylcholine receptor clustering at the postsynaptic membrane, a process vital for effective neuromuscular transmission. Mice lacking Dok-7 cannot form neuromuscular synapses or develop acetylcholine receptor clusters, leading to severe muscle weakness. Mutations in Dok-7 gene are associated with congenital myasthenic syndromes, inherited disorders characterized by muscle weakness and impaired neuromuscular transmission. anti-Dok-7 antibody (A-7) effectively detects this important protein, making Dok-7 (A-7) monoclonal antibody a valuable tool for researchers studying neuromuscular disorders and synaptic function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dok-7 Antibody (A-7) References:

1. Novel p62dok family members, dok-4 and dok-5, are substrates of the c-Ret receptor tyrosine kinase and mediate neuronal differentiation.  |  Grimm, J., et al. 2001. J Cell Biol. 154: 345-54. PMID: 11470823
2. Dok-6, a Novel p62 Dok family member, promotes Ret-mediated neurite outgrowth.  |  Crowder, RJ., et al. 2004. J Biol Chem. 279: 42072-81. PMID: 15286081
3. The muscle protein Dok-7 is essential for neuromuscular synaptogenesis.  |  Okada, K., et al. 2006. Science. 312: 1802-5. PMID: 16794080
4. Dok-7 mutations underlie a neuromuscular junction synaptopathy.  |  Beeson, D., et al. 2006. Science. 313: 1975-8. PMID: 16917026
5. Dok-7/MuSK signaling and a congenital myasthenic syndrome.  |  Yamanashi, Y., et al. 2008. Acta Myol. 27: 25-9. PMID: 19108574
6. Dok-7 activates the muscle receptor kinase MuSK and shapes synapse formation.  |  Inoue, A., et al. 2009. Sci Signal. 2: ra7. PMID: 19244212
7. The roles of Dok family adapters in immunoreceptor signaling.  |  Mashima, R., et al. 2009. Immunol Rev. 232: 273-85. PMID: 19909370
8. Dok-7 promotes slow muscle integrity as well as neuromuscular junction formation in a zebrafish model of congenital myasthenic syndromes.  |  Müller, JS., et al. 2010. Hum Mol Genet. 19: 1726-40. PMID: 20147321
9. Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L.  |  Hallock, PT., et al. 2010. Genes Dev. 24: 2451-61. PMID: 21041412
10. Overexpression of Dok-7 in skeletal muscle enhances neuromuscular transmission with structural alterations of neuromuscular junctions: Implications in robustness of neuromuscular transmission.  |  Eguchi, T., et al. 2020. Biochem Biophys Res Commun. 523: 214-219. PMID: 31848047
11. Identification of the downstream molecules of agrin/Dok-7 signaling in muscle.  |  Wang, B., et al. 2020. FASEB J. 34: 5144-5161. PMID: 32043676
12. Peroxisome proliferator-activated receptor γ coactivator 1α regulates downstream of tyrosine kinase-7 (Dok-7) expression important for neuromuscular junction formation.  |  Sugimoto, T., et al. 2024. Sci Rep. 14: 1780. PMID: 38245592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dok-7 Antibody (A-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Dok-7 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dok-7 Antibody (A-7) Specifications

Alternate Product Name: Dok-7

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 214-498

Epitope Target: Dok-7

Epitope Species: human

Product Reactivity/Detection: Dok-7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Dok-7 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin