Dok-3 Antibody (D-2): sc-365761

Dok-3 Antibody (D-2) is a mouse monoclonal IgG1 antibody that detects Dok-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dok-3 is a crucial adapter protein that plays a significant role in the negative regulation of immunoreceptor signaling in hematopoietic cells, particularly in B cells and macrophages. Dok-3 helps maintain cellular homeostasis and prevents overactivation of immune responses, which could lead to autoimmune disorders. Upon immunoreceptor-mediated activation, Dok-3 undergoes tyrosine phosphorylation, allowing interaction with key signaling molecules such as the 5′ inositol phosphatase SHIP and the protein tyrosine kinase Csk. This interaction modulates downstream signaling pathways, influencing cellular responses to external stimuli. Anti-Dok-3 antibody (D-2) serves as an invaluable tool for researchers studying immune signaling pathways and mechanisms of immune regulation.

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Dok-3 Antibody (D-2) References:

1. Dok-3, a novel adapter molecule involved in the negative regulation of immunoreceptor signaling.  |  Lemay, S., et al. 2000. Mol Cell Biol. 20: 2743-54. PMID: 10733577
2. Proteomic analysis of integrin alphaIIbbeta3 outside-in signaling reveals Src-kinase-independent phosphorylation of Dok-1 and Dok-3 leading to SHIP-1 interactions.  |  Senis, YA., et al. 2009. J Thromb Haemost. 7: 1718-26. PMID: 19682241
3. DOK3 negatively regulates LPS responses and endotoxin tolerance.  |  Peng, Q., et al. 2012. PLoS One. 7: e39967. PMID: 22761938
4. A physical interaction between the adaptor proteins DOK3 and DAP12 is required to inhibit lipopolysaccharide signaling in macrophages.  |  Peng, Q., et al. 2013. Sci Signal. 6: ra72. PMID: 23962980
5. Effects of expressing human T-cell leukemia virus type 1 (HTLV-I) oncoprotein Tax on DOK1, DOK2 and DOK3 gene expression in mice.  |  Ohsugi, T. 2017. J Vet Med Sci. 79: 935-938. PMID: 28302940
6. Elevated expression of DOK3 indicates high suppressive immune cell infiltration and unfavorable prognosis of gliomas.  |  Liu, X., et al. 2020. Int Immunopharmacol. 83: 106400. PMID: 32193105
7. Dok3 restrains neutrophil production of calprotectin during TLR4 sensing of SARS-CoV-2 spike protein.  |  Loh, JT., et al. 2022. Front Immunol. 13: 996637. PMID: 36172386
8. Porphyromonas gingivalis Activation of Tumor-Associated Macrophages via DOK3 Promotes Recurrence of Oral Squamous Cell Carcinoma.  |  Li, CX., et al. 2022. Med Sci Monit. 28: e937126. PMID: 36210538
9. Disrupting the Dok3-Card9 Interaction with Synthetic Peptides Enhances Antifungal Effector Functions of Human Neutrophils.  |  Loh, JT., et al. 2023. Pharmaceutics. 15: PMID: 37513967
10. Association of DOK3 and infiltrated tumor-associated macrophages with risk for the prognosis of Porphyromonas gingivalis-infected oral cancer: a 12-year data analysis of 200 patients from a tertiary teaching hospital, Urumqi, China.  |  Li, C., et al. 2024. BMC Cancer. 24: 534. PMID: 38671413

The preferred Dok-3 antibody is Dok-3 Antibody (F-7): sc-390007.