Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dnmt2 CRISPR/Cas9 KO Plasmid (m): sc-420034
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dnmt2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dnmt2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dnmt2 Antibody (D-9): sc-365001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dnmt2 CRISPR/Cas9 KO Plasmid (m) | sc-420034 | 20 µg | $397.00 |
Overview
Trdmt1 encodes Dnmt2, a highly conserved RNA cytosine-5 methyltransferase that predominantly modifies tRNAs (notably m5C at cytosine 38), influencing tRNA stability, decoding fidelity, and cellular stress responses. Through regulation of RNA methylation, Dnmt2 contributes to translation control and broader epitranscriptomic networks that intersect with RNA processing and genome maintenance pathways. Altered TRDMT1/DNMT2 activity has been linked to disrupted proteostasis and stress adaptation, with reported relevance to oncogenic phenotypes and neurobiological processes in model systems. In mouse, Trdmt1 is frequently studied for its role in RNA-mediated regulation of gene expression, cellular homeostasis, and context-dependent responses to environmental and metabolic stressors.
Dnmt2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Trdmt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Trdmt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Trdmt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dnmt2 protein expression.
This CRISPR knockout system enables efficient generation of Trdmt1-deficient cell models for investigation of Dnmt2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Trdmt1 exon(s) critical for Dnmt2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Trdmt1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dnmt2 CRISPR/Cas9 KO Plasmid (m) and Dnmt2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Trdmt1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dnmt2 HDR Plasmid (m) and Dnmt2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Trdmt1 homology arms to support homology-directed repair at defined Trdmt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.