Dnmt2 Antibody (D-9): sc-365001

Dnmt2 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dnmt2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dnmt2 antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dnmt2 plays a crucial role in the methylation of newly integrated retroviral DNA, which is essential for regulating gene expression and maintaining genomic stability. Unlike Dnmt1, which is primarily responsible for maintaining DNA methylation patterns during cell division, Dnmt2 is expressed at lower levels in adult tissues and lacks the large N-terminal regulatory domain found in Dnmt1. This unique structure allows Dnmt2 to participate in distinct methylation processes, particularly in the context of retroviral integration, thereby influencing the epigenetic landscape of the host genome. Understanding Dnmt2 function is vital, as Dnmt2 contributes to the broader mechanisms of gene regulation and cellular response to environmental changes, making Dnmt2 a significant target for research in epigenetics and virology.

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Dnmt2 Antibody (D-9) References:

1. DNA methyltransferase is actively retained in the cytoplasm during early development.  |  Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
2. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b.  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
3. DNA methyltransferase Dnmt1 associates with histone deacetylase activity.  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
4. Transcriptional regulation of the human DNA Methyltransferase (dnmt1) gene.  |  Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
5. Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism.  |  Jurkowski, TP., et al. 2008. RNA. 14: 1663-70. PMID: 18567810
6. Dnmt2-dependent methylomes lack defined DNA methylation patterns.  |  Raddatz, G., et al. 2013. Proc Natl Acad Sci U S A. 110: 8627-31. PMID: 23641003
7. The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu.  |  Shanmugam, R., et al. 2014. Nucleic Acids Res. 42: 6487-96. PMID: 24711368
8. RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines.  |  Betlej, G., et al. 2022. J Bone Oncol. 36: 100448. PMID: 35942470
9. tRNA methyltransferase DNMT2 promotes hepatocellular carcinoma progression and enhances Bortezomib resistance through inhibiting TNFSF10.  |  Lai, J., et al. 2025. Cell Signal. 127: 111533. PMID: 39617358
10. DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe.  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
11. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells.  |  Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
12. Cytosine methylation and mammalian development.  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dnmt2 Antibody (D-9) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Dnmt2 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dnmt2 Antibody (D-9) Specifications

Alternate Product Name: DNMT

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 121-391

Epitope Target: Dnmt2

Epitope Species: human

Product Reactivity/Detection: Dnmt2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Dnmt2 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin