DnaJB14 Antibody (D-6): sc-515383
- DnaJB14 Antibody (D-6) is a mouse monoclonal IgG1 κ DnaJB14 antibody provided at 200 µg/ml
- raised against amino acids 303-379 mapping at the C-terminus of DnaJB14 of human origin
- DnaJB14 Antibody (D-6) is recommended for detection of DnaJB14 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DnaJB14 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DnaJB14 Antibody (D-6) for WB applications. These reagents are now offered in bundles with DnaJB14 Antibody (D-6) (see ordering information below).
DnaJB14 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects DnaJB14 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DnaJB14 antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DnaJB14, a member of the DnaJ family, plays a crucial role in cellular protein homeostasis by functioning as a co-chaperone that assists in the proper folding of nascent polypeptides and the refolding of misfolded proteins. DnaJB14 is primarily located in the endoplasmic reticulum and is essential for maintaining cellular health, particularly under stress conditions where protein misfolding is prevalent. DnaJB14 interacts with the HSP70 chaperone machinery, facilitating the ATP hydrolysis necessary for protein folding and preventing aggregation, which is vital for cellular function and survival. The presence of a J domain in DnaJB14 is indicative of chaperone activity, and two alternatively spliced isoforms allow for functional diversity in response to various cellular conditions. DnaJB14 (D-6) monoclonal antibody enables researchers to gain insights into the mechanisms of protein folding and the cellular stress response, making this tool invaluable for studies in molecular biology and disease research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DnaJB14 Antibody (D-6) References:
- Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profiling. | Stewart, GR., et al. 2004. Tuberculosis (Edinb). 84: 180-7. PMID: 15207487
- The C-terminal (331-376) sequence of Escherichia coli DnaJ is essential for dimerization and chaperone activity: a small angle X-ray scattering study in solution. | Shi, YY., et al. 2005. J Biol Chem. 280: 22761-8. PMID: 15849180
- DnaJA4 is a SREBP-regulated chaperone involved in the cholesterol biosynthesis pathway. | Robichon, C., et al. 2006. Biochim Biophys Acta. 1761: 1107-13. PMID: 16950652
- The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. | Genevaux, P., et al. 2007. Mol Microbiol. 66: 840-57. PMID: 17919282
- DnaJ recruits DnaK to protein aggregates. | Acebrón, SP., et al. 2008. J Biol Chem. 283: 1381-1390. PMID: 17984091
- Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12. | Saito, H. and Uchida, H. 1978. Mol Gen Genet. 164: 1-8. PMID: 360036
- Identification of the E. coli dnaJ gene product. | Georgopoulos, CP., et al. 1980. Mol Gen Genet. 178: 583-8. PMID: 6446654
- Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli. | Tomoyasu, T., et al. 1998. Mol Microbiol. 30: 567-81. PMID: 9822822
- Interaction of the Hsp70 molecular chaperone, DnaK, with its cochaperone DnaJ. | Suh, WC., et al. 1998. Proc Natl Acad Sci U S A. 95: 15223-8. PMID: 9860950
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DnaJB14 Antibody (D-6) | sc-515383 | 200 µg/ml | $316.00 | |||
DnaJB14 Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-528032 | 200 µg Ab; 10 µg BP | $354.00 | |||
DnaJB14 Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-533405 | 200 µg Ab; 20 µg BP | $354.00 | |||
DnaJB14 Antibody (D-6) AC | sc-515383 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DnaJB14 Antibody (D-6) HRP | sc-515383 HRP | 200 µg/ml | $316.00 | |||
DnaJB14 Antibody (D-6) FITC | sc-515383 FITC | 200 µg/ml | $330.00 | |||
DnaJB14 Antibody (D-6) PE | sc-515383 PE | 200 µg/ml | $343.00 | |||
DnaJB14 Antibody (D-6) Alexa Fluor® 488 | sc-515383 AF488 | 200 µg/ml | $357.00 | |||
DnaJB14 Antibody (D-6) Alexa Fluor® 546 | sc-515383 AF546 | 200 µg/ml | $357.00 | |||
DnaJB14 Antibody (D-6) Alexa Fluor® 594 | sc-515383 AF594 | 200 µg/ml | $357.00 | |||
DnaJB14 Antibody (D-6) Alexa Fluor® 647 | sc-515383 AF647 | 200 µg/ml | $357.00 | |||
DnaJB14 Antibody (D-6) Alexa Fluor® 680 | sc-515383 AF680 | 200 µg/ml | $357.00 | |||
DnaJB14 Antibody (D-6) Alexa Fluor® 790 | sc-515383 AF790 | 200 µg/ml | $357.00 |