Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DnaJA4 CRISPR/Cas9 KO Plasmid (h): sc-406743
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DnaJA4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DnaJA4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DnaJA4 Antibody (LL2): sc-100714
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DnaJA4 CRISPR/Cas9 KO Plasmid (h) | sc-406743 | 20 µg | $397.00 |
Overview
DNAJA4 encodes DnaJA4, a member of the Hsp40 (DnaJ) co-chaperone family that binds unfolded client proteins and stimulates Hsp70 ATPase activity to promote proper folding, refolding, and triage of misfolded proteins. Through these chaperone-driven quality control processes, DnaJA4 contributes to proteostasis networks that intersect with cellular stress responses and protein turnover pathways. Dysregulated chaperone activity can influence the stability and function of signaling proteins, impacting pathways that govern cell survival, differentiation, and stress adaptation. Altered proteostasis capacity is broadly relevant to disease biology, including contexts where aberrant protein folding, aggregation, or stress signaling supports pathological phenotypes.
DnaJA4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAJA4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAJA4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAJA4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DnaJA4 protein expression.
This CRISPR knockout system enables efficient generation of DNAJA4-deficient cell models for investigation of DnaJA4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAJA4 exon(s) critical for DnaJA4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAJA4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DnaJA4 CRISPR/Cas9 KO Plasmid (h) and DnaJA4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAJA4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DnaJA4 HDR Plasmid (h) and DnaJA4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAJA4 homology arms to support homology-directed repair at defined DNAJA4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.