DnaJA4 Antibody (LL2): sc-100714

DnaJA4 Antibody (LL2) is a mouse monoclonal IgG1 kappa light chain antibody that detects DnaJA4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-DnaJA4 antibody (LL2) is available as a non-conjugated monoclonal isotype antibody. The DnaJ family, to which DnaJA4 belongs, is one of the largest chaperone families and is characterized by diverse cellular localization and functions, primarily defined by the presence of the J domain. DnaJA4 plays a crucial role in regulating the cholesterol biosynthesis pathway, acting as a SREBP-regulated chaperone. DnaJA4 is essential for maintaining cellular homeostasis, particularly in lipid metabolism, as DnaJA4 assists in proper folding and functioning of other proteins involved in cholesterol synthesis. DnaJA4 interaction with HSP 70 enhances ATP hydrolysis, which is vital for chaperone activity in preventing protein aggregation and facilitating protein degradation. DnaJA4 contains cysteine-rich regions that form zinc finger motifs, contributing to peptide binding domain and overall chaperone function. DnaJA4′s ability to mediate these processes underscores importance in cellular stress responses and metabolic regulation, making DnaJA4 a significant target for research in various diseases related to cholesterol metabolism and protein misfolding.

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DnaJA4 Antibody (LL2) References:

1. Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profiling.  |  Stewart, GR., et al. 2004. Tuberculosis (Edinb). 84: 180-7. PMID: 15207487
2. The C-terminal (331-376) sequence of Escherichia coli DnaJ is essential for dimerization and chaperone activity: a small angle X-ray scattering study in solution.  |  Shi, YY., et al. 2005. J Biol Chem. 280: 22761-8. PMID: 15849180
3. DnaJA4 is a SREBP-regulated chaperone involved in the cholesterol biosynthesis pathway.  |  Robichon, C., et al. 2006. Biochim Biophys Acta. 1761: 1107-13. PMID: 16950652
4. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions.  |  Genevaux, P., et al. 2007. Mol Microbiol. 66: 840-57. PMID: 17919282
5. DnaJ recruits DnaK to protein aggregates.  |  Acebrón, SP., et al. 2008. J Biol Chem. 283: 1381-1390. PMID: 17984091
6. Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12.  |  Saito, H. and Uchida, H. 1978. Mol Gen Genet. 164: 1-8. PMID: 360036
7. Identification of the E. coli dnaJ gene product.  |  Georgopoulos, CP., et al. 1980. Mol Gen Genet. 178: 583-8. PMID: 6446654
8. Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli.  |  Tomoyasu, T., et al. 1998. Mol Microbiol. 30: 567-81. PMID: 9822822
9. Interaction of the Hsp70 molecular chaperone, DnaK, with its cochaperone DnaJ.  |  Suh, WC., et al. 1998. Proc Natl Acad Sci U S A. 95: 15223-8. PMID: 9860950

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DnaJA4 Antibody (LL2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DnaJA4 Antibody (LL2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DnaJA4 Antibody (LL2) Specifications

Alternate Product Name: DnaJA4

Product Clone Name: LL2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-398

Epitope Target: DnaJA4

Epitope Species: human

Product Reactivity/Detection: DnaJA4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DnaJA4 Antibody (LL2): N/A